| 豚鼠心房肌细胞的分离及其离子流记录 |
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| 引用本文： | 杨向军,蒋文平.豚鼠心房肌细胞的分离及其离子流记录[J].心电学杂志,1997(3). |
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| 作者姓名： | 杨向军 蒋文平 |
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| 作者单位： | 苏州医学院附属第一医院心内科 215006 |
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| 摘 要： | 为探讨豚鼠心房肌细胞的分离方法及其离子流记录,采用酶解技术分离豚鼠心房肌细胞,应用膜片钳全细胞记录技术,记录细胞膜L型钙流(L-I_(Ca))及延迟整流性钾流(I_K)。结果获得了具正常电生理活性的单个细胞及高阻封接的形成,成功记录了L-型钙流和延迟整流性钾流。认为本方法分离的单个心房肌细胞具正常的电生理活性,可用于研究心房肌细胞离子通道活性。
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| 关 键 词： | 心房肌细胞 膜片钳 离子流 |
Isolation of single guinea pig atrial myocyte and its ionic current recording |
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| Yang Xiang-jun,Jiang Wen-ping. Cardiovascular Laboratory,First Affiliated Hospital,Suzhou Medical College,Suzhou.Isolation of single guinea pig atrial myocyte and its ionic current recording[J].Journal of Electrocardiology(China),1997(3). |
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| Authors: | Yang Xiang-jun Jiang Wen-ping Cardiovascular Laboratory First Affiliated Hospital Suzhou Medical College Suzhou |
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| Institution: | Yang Xiang-jun,Jiang Wen-ping. Cardiovascular Laboratory,First Affiliated Hospital,Suzhou Medical College,Suzhou,215006 |
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| Abstract: | In order to study methed for isolating single atrial myocyte and its ionic current recondihg, single guinea-pig atrial myocyte obtained by enzyme isolated method and patch-clamp technique was adopted to record L-Ica and IK. As a result single guinea-pig atrial myocyte with normal electrophysiologic activity was obtained and its L-Ica and IK were successfully recorded with patch-clamp technique. Authors have come to conclussion that enzyme isolated method is a success in obtaining single guinea-pig atrial myocyte with normal electrophysiologic activity, and study of ionic channel activity with patch-clamp technique will become possible. |
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| Keywords: | Single atrial myocyte Patch-clamp Ion- |
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