外周血双色荧光原位杂交方法监测慢性粒细胞性白血病患者干扰素治疗前后bcr／abl融合基因的变化

目的：研究外周血双色荧光原住杂交(D-FISH)在慢性粒细胞性白血病(CML)患者干扰素治疗效果监测方面的应用价值。方法：用D-FISH方法对18例CML患者干扰素治疗前后的外周血36份样本进行bcr／abl融合基因检测，并且与骨髓D-FISH方法检测的结果进行比较。结果：治疗后外周血间期核细胞中bcr／abl融合基因的检出率较治疗前有所下降，并且外周血中的检出率比同一患者骨髓中的检出率要低。结论：D-FISH方法可以简便、灵敏、可靠地对CML患者干扰素治疗前后的外周血进行疗效监测，使得从取材上更加方便。

MONTORITN THE BCR/ABL FUSION ON PERIPHERAL BLOOD OF CHRONIC MYELOID LEUKEMIA PATIENTS TREATED BY INTERFERON WITH DUOL-COLOR FLUORESCENCE IN SITU HYBRIDIZATION

Objective:To investigate the value of duol color fluorescence in situ hybridization (D FISH) in monitoring the response to IFN therapy in chronic myelid leukemia (CML) patients on peripheral blood.Methods:Monitoring the positive cells proportion of the bcr/abl fusion gene on peripheral blood of CML patients before and after treated by interferon, and compared with the results of the bone marrow.Results:The bcr/abl fusion gene of the 18 atients are all decreased after the interferon therapy on peripheral blood, and less than in the bone marrow.Conclusion:The D FISH method on the peripheral blood is sensitive, credible and quick to monitoring the response of the interferon treatment of CML.It is more convenienter.