外周血EGFR mRNA在膀胱癌中的表达及在微转移中的意义

目的：探讨表皮生长因子受体（Epidermal growth factor receptor,EGFR）mRNA在膀胱移行细胞癌（transitional cell carcinoma,TCC）患者外周血中的表达及意义。方法：收集62例膀胱移行细胞癌患者外周静脉血及癌组织标本,提取总RNA,行RT-PCR,检测EGFR mRNA的表达。25例健康人外周静脉血标本作为对照。结果：62例膀胱TCC组织标本中,EGFR mRNA在组织中平均阳性率72.6%（45/62）。在Tis-1、T2、T3、T4期膀胱TCC患者组织中EGFR mRNA阳性率分别为57.1%、62.5%、85.7%、100%;在Tis-1、T2、T3、T4期膀胱TCC患者外周血中EGFR mRNA阳性率分别为14.3%、25.0%、78.6%、90.9%。在组织和外周血中低分期（Tis-1、T2）与高分期（T3、T4）膀胱TCC表达EGFR mRNA阳性率差异有显著性（P＆lt;0.01）。在25例健康人外周静脉血中均未发现EGFR mRNA的表达。结论：膀胱TCC组织及外周血中EGFR mRNA表达阳性率与肿瘤分期、是否转移呈相关性。RT—PCR法检测膀胱TCC患者外周血EGFRmRNA有望成为检测膀胱肿瘤细胞循环内微转移的有效方法。

Expression of Epidermal Growth Factor Receptor mRNA of Peripheral Blood in the Bladder Transitional Cell Carcinoma and Its Micrometastasis

Objective： To explore the expression of epidermal growth factor receptor （EGFR） mRNA in peripheral blood of patients with bladder transitional cell cancer（ TCC ） and its significance. Methods： Peripheral vein blood and tumor tissue samples of 62 patients with bladder TCC （ group TCC） were collected and peripheral blood of 25 healthy subjects were collected as negative control （ group C）. The expression of EGFR mRNA was detected by RT-PCR. Results： The positive rates of EGFR mRNA expression averaged 72.6% （45/62） including 57.1% ,62.5% ,85.7% ,and 100 % in tissue samples and 14.3% ,25.0% ,78.6%, and 90.9% in peripheral blood samples from group TCC of stages of Tis-1, T2,33, and T4 respectively. There was a significant difference of EGFR mRNA expression in tissue and blood samples of TCC between stages Tis-1 ,T2 and stages T3, T4 （ P 〈 0. 01 ） . EGFR mRNA was not detected in the 25-healthy people. Conclusion： Positive rates of EGFR mRNA expression in samples of tissue and peripheral blood were correlated with the staging and grading, and metastasis of TCC. RT-PCR might be one of the effective methods for detecting micrometastasis in circulating of bladder tumor cells.