Application of multiplex PCR in genotyping of hepatitis B virus prevailing in Guangdong Province of China] |
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Authors: | Jie Yang Lin Dai Ya-Bing Guo Shou-Chang Yang Yan-Jun Wang Kang-Xian Luo |
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Institution: | Department of Infectious Diseases, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China. yangjie@fimmu.edu.cn |
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Abstract: | OBJECTIVE: To establish a convenient method for the genotyping of hepatitis B virus (HBV) using multiplex PCR. METHOD: Based on the alignment of 114 complete nucleotide sequences of HBV DNA belonging to different genotypes, acquired from the GenBank, genotype-specific sequences were identified according to which 6 pairs of primers were designed corresponding to each genotype. Subsequent genotyping of HBV was performed using these primers that were added, either alone or in conjunction with others, into a multiplex PCR reaction tube, and HBV genotype was determined according to the length of amplified DNA. RESULT: The genotyping result of multiplex PCR was consistent with that produced by PCR- restriction fragment length polymorphism as established by Lindh. We found in this study that among the HBV carriers in the vicinities Guangzhou of City, about 45% belonged to B genotype, 38.75% to C genotype and 16.75% to D genotype. CONCLUSION: This multiplex PCR method is simple, convenient and more differential. |
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