喉鳞状细胞癌中PIN1基因扩增及表达异常

目的：研究PIN1基因在喉癌组织中的表达情况。方法：采用差异PCR、核型分析方法检测喉鳞癌及癌旁对照组织中PIN1基因扩增情况;采用RT-PCR检测同批组织中PIN1 mRNA表达情况;采用免疫组织化学、Western Blot方法检测PIN1蛋白表达情况。结果：与癌旁对照组织相比,40例喉癌组织中有9例（9/40,22.5%）存在PIN1基因扩增,27例（27/40,67.5%）PIN1 mRNA过表达,26例（26/40,65%）PIN1蛋白过表达（P〈0.05）。40例中17例原代培养成功,可制备中期染色体标本,其中3例（3/17,17.65%）存在19号染色体增加。结论：喉鳞状细胞中PIN1基因拷贝数增加,PIN1 mRNA和蛋白质过表达,可能与喉癌发生发展有关。

PIN1 gene amplification and overexpression in human laryngeal squamous cell carcinoma

OBJECTIVE:To investigate the amplification and overexpression of PIN1 gene in human laryngeal squamous cell carcinoma. METHODS: PIN1 gene amplification(increase of gene copy number) was detected by differential PCR and Karyotype analysis. PIN1 mRNA was evaluated by RT-PCR. Furthermore,immunohistochemistry and Western Blot were employed to detect the PIN1 protein. RESULTS: Compared with nomal control,PIN1 gene amplification rate was 22.5%(9/40),while 17.65%(3/17) was found to have an increase of chromosome 19; the rate of mRNA overexpression was 67.5%(27/40). PIN1 protein was obviously upregulated,the ratio was 65%(26/40)(P<0.05). CONCLUSION: PIN1 gene copy increased,with mRNA and protein overexpression in laryngeal carcinoma. This fact may be relevant to laryngeal cancer development.