| EGF联合KGF促进人角膜上皮细胞生长 |
| |
| 引用本文： | 龚向明,钟兴武. EGF联合KGF促进人角膜上皮细胞生长[J]. 眼科学报, 1996, 0(2) |
| |
| 作者姓名： | 龚向明 钟兴武 |
| |
| 作者单位： | 卫生部眼科学实验室，中山医科大学眼科中心 广州 510060 |
| |
| 摘 要： | 目的:寻找促进角膜上皮损伤修复,治疗持续性角膜上皮缺损的有效药物方法:用~3H—胸腺嘧啶核苷(~3H—TDR)掺入及液体闪烁技术,观察外源性表皮生长因子(EGF)联合角蛋白细胞生长因子(KGF)对体外培养的角膜上皮细胞DNA合成的影响,并计算细胞倍增时间。结果:10ng/mlEGF,10ng/mlKGF单独或联合应用均有明显促进人角膜上皮细胞DNA合成的作用(与对照组比较 P<0.01),联合用药,作用更强(P<0.05)。应用EGF与KGF明显缩短了细胞倍增时间。结论:外源性EGF与KGF对体外培养的人角膜上皮细胞有明显的促细胞增生作用,联合用药,效果更佳。表明EGF与KGF具有应用于临床,促进角膜上皮损伤修复的可能性。眼科学报1996; 12:107-109。
|
| 关 键 词： | 表皮生长因子 角蛋白细胞生长因子 人角膜上皮细胞 细胞培养 细胞增生 |
Effect of Combining EGF with KGF on Proliferation of Human Corneal Epithelial Cells |
| |
| Xiangming Gong,Xingwu ZhongZhongshan Ophthalmic Center,Sum Yat-sen University of Medical Sciencesand National Ophthalmological Laboratory of Public Health,Guangzhou ,China. Effect of Combining EGF with KGF on Proliferation of Human Corneal Epithelial Cells[J]. Eye science, 1996, 0(2) |
| |
| Authors: | Xiangming Gong Xingwu ZhongZhongshan Ophthalmic Center Sum Yat-sen University of Medical Sciencesand National Ophthalmological Laboratory of Public Health Guangzhou China |
| |
| Affiliation: | Xiangming Gong,Xingwu ZhongZhongshan Ophthalmic Center,Sum Yat-sen University of Medical Sciencesand National Ophthalmological Laboratory of Public Health,Guangzhou 510060,China |
| |
| Abstract: | Objective :To select effective durgs for enhancing corneal epithelial wound healingand treating persistent corneal epithelial defect.Methods-. Epidermal growth factor (EGF) and keratinocyte growth factor(KGF)were added to cultures of human corneal epithelial cells and DNA synthesis incorneal epithelial cells were measured with [3H] - thymidine incorporation andliquid scintillation techniques. The cells were counted in a hemocytometer todetermine the doubling time.Results: 10ng/ml EGF,10ng/ml KGF enhanced cellular proliferation effectively(P<0. 01). EGF in combination with KGF,the enhancement was more effective(P<0. 05) and reduced the doubling time.Conclusions : Our results suggest that exogenous EGF with KGF can stimulateproliferation of human corneal epithelial cells and it may be of potential useclinically for treatment of persistent corneal epithelial use clinically for treatmentof persistent corneal epithelial defect. Eye Science 1996; 12: 107- 109. |
| |
| Keywords: | epidermal growth factor keratinocyte growth factor human corneal epithelial cell cell culture cell proliferation |
| 本文献已被 CNKI 等数据库收录！ |
