血吸虫卵肉芽肿分离培养及培养上清液中抗体检测的初步研究

自感染25条日本血吸虫尾蚴6wk后的小鼠肝脏分离虫卵肉芽肿进行体外培养,用ELISA方法检测培养上清液中的SEA特异性抗体。感染鼠肝内分离到的虫卵肉芽肿大小不一,其直径的频数分布呈正偏态分布。在某些小鼠个体间虫卵肉芽肿大小也有些差异。虫卵肉芽肿在培养时宜根据肉芽肿的容积按1:10的比例进行调整,培养持续时间在48h以内即可。虫卵肉芽肿在体外培养时能分泌特异性抗体,提示抗体与虫卵肉芽肿的形成有关。

PRELIMINARY STUDIES ON ISOLATION AND CULTURE OF EGG GRANULOMAS OF SCHISTOSOMA JAPONICUM FROM INFECTED MICE AND DETECTION OF ANTIBODIES IN SUPERNATANT OF CULTURE MEDIUM

Egg granulomas were isolated from livers of mice 6 weeks after inoculation with 25 cer-cariae of Schistosorna japonicum and incubated in vitro. The supernatant of culture was tested for SEA-specific antibodies by means of ELISA. The size of egg granulonas varied and the freguency distribution of mean granuloma diameter showed positive skewness ditribution. The size of granulomas isolated from different infected mice also varied. It was appropriate to adjust the volume of egg granuloma and the culture medium in the ratio of 1 : 10 and the time for culture should not be more than 48 hr. The fact that the egg granulomas could secret specific antibodies while being incubated in vitro, indicated that the antibodies were related to the formation of egg granulomas.