硫磺熏蒸当归二氧化硫残留与内在质量变化相关性

目的:以探讨经硫磺熏蒸后当归中二氧化硫残留量与硫熏诱导的含硫衍生物量的相关性为目的。方法:采用2010年版《中国药典》第一增补本中的方法对二氧化硫残留量进行动态检测;采用UPLC-QTOF-MS/MS对不同硫磺熏蒸时间当归中的含硫衍生物进行半定量测定;将二氧化硫残留量与含硫衍生物测定结果进行统计学分析。结果:在480 min内,随着硫磺熏蒸时间的增加,二氧化硫残留量呈现先升高后略降,再趋于平缓的趋势;水煎液中含硫衍生物的含量明显高于甲醇提取液,并随硫熏时间延长先升高后缓慢降低。结论:硫磺熏蒸当归二氧化硫残留与含硫衍生物含量无显著相关性,仅以二氧化硫残留量作为硫磺熏蒸当归检控指标值得商榷。

Correlation Between Sulfur Dioxide Residue and Quality Change of Sulfur-fumigated Angelica Sinensis Radix

Objective: To investigate the correlation between sulfur dioxide residue and the content of sulfur-containing derivatives in Angelica Sinensis Radix after sulfur-fumigation. Method: The method in Chinese Pharmacopoeia (2010 version, the first supplement) was used to determine the dynamic change of sulfur dioxide residue; UPLC-QTOF-MS/MS method was used to quantify the sulfur-containing derivatives at different sulfur-fumigation time points. The determination results of sulfur dioxide residue and sulfur-containing derivatives were statistically analyzed. Result: With the increase of sulfur-fumigation duration, sulfur dioxide residue first increased, then reduced and became flat within 480 min. The content of sulfur-containing derivatives in decoction was obviously higher than that in methanol extract, and first increased and then slowly reduced with the increase of sulfur-fumigation duration. Conclusion: There is no clear correlation between sulfur dioxide residue and sulfur-containing derivatives in sulfur-fumigated Angelica Sinensis Radix. Therefore, sulfur dioxide residue alone might be inadequate to comprehensively evaluate the quality control of sulfur-fumigated Angelica Sinensis Radixs.