肿瘤抑制基因p16~(INK4A)在小鼠早孕子宫内膜中的表达

目的:初步探讨p16INK4A在胚胎着床过程中的作用。方法:采用实时FQ-PCR和免疫组织化学技术分别检测未孕(d0)及孕d2、d3、d4、d5、d6、d7小鼠子宫内膜p16INK4AmRNA及其蛋白的表达。结果:实时FQ-PCR显示妊娠子宫内膜组织p16INK4A的mRNA表达高于未妊娠的子宫内膜组织,且随着妊娠天数的增加呈现逐渐增强的趋势,到孕d5达到最高。免疫组化显示p16INK4A蛋白在子宫内膜的表达及规律与实时FQ-PCR的结果一致。结论:小鼠胚胎着床过程中p16INK4A诱导的小鼠子宫内膜上皮细胞的凋亡可能是胚胎着床的重要机制之一。

Expression of p16INK4A in Mouse Endometrium during Early Pregnancy

Objective:To investigate the effect of p16INK4A gene on embryo implantation during early pregnancy.Methods:Real-time fluorescence quantitative PCR(FQ-PCR) and immunohistochemistry were used to detect the p16INK4A mRNA and protein in endometrial tissues of unpregnancy(d 0) and pregnant mice from d 2 to d 7,respectively.Results:Real-time FQ-PCR result showed that the p16INK4A mRNA expression in pregnant group was higher than that in non-pregnant group.It showed that p16INK4A mRNA expression was increased with the progression of pregnancy and reached the peak on d 5 of pregancy.Immunohistochemical analysis showed that p16 positive signals presented mainly in luminal epithelial cells and less in stromal cells with the pattern parallel to the FQ-PCR.Conclusion:Probably the apoptosis of epithelial cell in endometrium induced by p16INK4A could be one of the mechanisms involved in penetrating to the epithelial barrier by embryos.