COX-2、MMP-2在直肠癌中的表达及其相关性分析

目的探讨人直肠癌中环氧化酶-2（COX-2）、基质金属蛋白酶-2（MMP-2）的形态表达特点及其与侵袭和转移的关系。方法选取直肠癌组织46例，正常直肠组织12例，分别应用COX-2单克隆抗体和MMP-2单克隆抗体进行免疫组织化学染色，观察COX-2、MMP-2的表达水平，结果进行统计学处理。结果COX-2在直肠癌中的阳性表达率为63．04％，在正常直肠组织中阳性表达率为25％，差别有统计学意义（P〈0．05）；其表达程度与直肠癌的临床分期和转移有关（P〈0．05），而与其组织学类型及分级无关（P〉0．05）。MMP．2在直肠癌中的表达率为67．39％，在正常直肠组织中表达率为25％，差异有统计学意义（P〈0．05）；其表达程度与直肠癌的分级、临床分期和转移有关（P〈0．05），与其组织学类型无关（P〉0．05）。结论COX-2、MMP-2在直肠癌组织中均呈过表达，其表达程度具有相关性。COX-2、MMP．2的表达与直肠癌的发生、侵袭和转移密切相关。

Expression of COX-2 and MMP-2 in Rectal Carcinoma and Their Relationship

Objective To study the expression of cyclooxygenase-2 （COX-2） and matrix metalloproteinase-2 （MMP-2） in rectal carcinoma and their relationship with tumor cell invasion and metastasis. Methods Using monoclonal antibodies for COX-2 and MMP-2, the expression of COX-2 and MMP-2 were detected by means of immun0histochemistry in 46 cases of rectal carcinoma and 12 cases normal rectal tissue as controls. The results were analysis statistically. Results The positive expression rate of COX-2 and MMP-2 in rectal carcinoma and normal rectal tissues was 63.04% and 25% （P 〈 0.05 ） ,67.39% and 25% （ P 〈 0.05 ）, respectively. The expression of COX-2 was intimately related to Dukes stage and metastasis of rectal carcinoma （ P 〈 0.05 ） ,but not related to subset of cells and pathological grade of rectal carcinoma（ P 〉0.05 ） ;while MMP-2 was closely related to pathological grade, Dukes stage and metastasis of rectal carcinoma（ P 〈 0.05 ） ,not related to subset of cells of rectal carcinoma （ P 〉 0.05 ）. Conclusion COX-2 and MMP-2 are over-expressed in rectal carcinoma with certain correlation. The over-expres- Sion of COX-2 and MMP-2 was significantly correlated to its invasion and metastasis and metastasis.