端粒酶在眼睑皮脂腺癌中表达的意义

目的　探讨端粒酶与端粒酶RNA(humantelomeraseRNA ,hTR)在睑板腺癌中的表达及其与肿瘤生物学行为的关系。方法　用端粒酶重复扩增技术法检测 12例冰冻保存睑板腺癌组织标本中端粒酶活性 ;用原位杂交法检测 5 5例睑板腺癌石蜡切片中hTR的表达 ,并用免疫组化EnVision法检测肿瘤增殖指数Ki 6 7的表达。结果　 12例睑板腺癌组织标本中 ,端粒酶活性程度不同 ;hTR在睑板腺癌细胞内表达 ,其阳性表达率为 85 5 % (47/5 5 ) ,而癌旁组织中hTR表达阴性 ;hTR的表达水平随睑板腺癌分化程度的降低而呈上升的趋势 (χ2 =17 6 2 1,P <0 0 0 1) ;hTR表达与睑板腺癌肿瘤增殖指数Ki 6 7表达及睑板腺癌复发无关。结论　hTR在睑板腺癌变过程中具有调节作用 ,hTR阳性表达可作为判断睑板腺癌恶性程度的标记物。

Telomerase expression in tarsal gland carcinoma

OBJECTIVE: To investigate the roles of telomerase activity (TA) and human telomerase RNA (hTR) in tarsal gland carcinoma. METHODS: The telomerase repeated amplification protocol (TRAP) was used to demonstrate telomerase activity in 12 tarsal gland carcinomas. In situ hybridization (ISH) was used to demonstrate the expression of hTR in 55 cases of paraffin-embedded tarsal gland carcinoma, and the results were compared with the proliferative index determined by Mib-1 immunolabeling, histological patterns and recurrence of the tumor. RESULTS: The telomerase activity was revealed in 12 tarsal gland carcinomas with different degrees. The positive expression of hTR was 85.5% (47/55) and observed in tumor cells, but not in the adjacent tissues. The expression of hTR was correlated with the proliferative activity (as assessed by Mib-1 immunolabelling, r = 0.942, P < 0.001) and the differentiation of tarsal gland carcinoma (chi(2) = 17.621, P < 0.001), but no significant relationship with tumor recurrence. The level of hTR expression had a gradually raised tendency along with the decrease of differentiation of tarsal gland carcinoma. CONCLUSION: The results suggest that up-regulation of telomerase expression play some roles in tarsal gland carcinogenesis, and the expression of hTR be a useful marker for malignant degree of tarsal gland carcinoma.