| 内皮抑素对宫颈癌细胞生长抑制作用的初步研究 |
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| 引用本文： | 程丽,李文辉,刘梅梅,隋丽华.内皮抑素对宫颈癌细胞生长抑制作用的初步研究[J].现代肿瘤医学,2007,15(8):1065-1068. |
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| 作者姓名： | 程丽 李文辉 刘梅梅 隋丽华 |
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| 作者单位： | 1. 哈尔滨医科大学附属第三医院,黑龙江,哈尔滨,150040
2. 哈尔滨医科大学附属第二医院,黑龙江,哈尔滨,150086 |
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| 摘 要： | 目的:探讨内皮抑素对人宫颈癌Hela细胞生长的抑制作用及其作用机制。方法:用MTT法检测细胞生长;用透射电镜观察细胞凋亡;用免疫细胞化学、RT-PCR及Western blot法检测细胞中bcl-2和bax蛋白及mRNA的表达。结果:内皮抑素抑制Hela细胞增殖;能诱导Hela细胞凋亡;而对细胞中bcl-2和bax蛋白及mRNA的表达无明显影响。结论:人内皮抑素具有抑制宫颈癌Hela细胞生长的作用,其作用机制可能与诱发细胞凋亡相关。
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| 关 键 词： | 宫颈癌细胞 内皮抑素 凋亡 |
| 文章编号： | 1672-4992-（2007）08-1065-04 |
| 修稿时间： | 2006-10-30 |
Inhibition of Endostatin on the growth of uterine cervix cancer cell Hela |
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| CHENG Li,LI Wen-hui,LIU Mei-mei,SUI Li-hua.Inhibition of Endostatin on the growth of uterine cervix cancer cell Hela[J].Journal of Modern Oncology,2007,15(8):1065-1068. |
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| Authors: | CHENG Li LI Wen-hui LIU Mei-mei SUI Li-hua |
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| Institution: | 1The Third Affiliated Hospital, Harbin Medical University, Heilongjiang 150040, China ;2 The Second Affiliated Hospital, Harbin Medical University, Heilongjiang 150086, China. |
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| Abstract: | Objective:To evaluate the inhibitory effect of endostatin on uterine cervix cancer cell line Hela and to investigate the possible mechanism of the inhibition.Methods:The effect of endostatin on Hela cell proliferation was studied by means of MTT.The cell apoptosis was detected by transmission electron microscopy.Then bcl-2 and bax expressions were measured in Hela cells treated by endostatin by immunocytochemistry,RT-PCR and Western blot method.Results:Endostatin inhibited Hela cell proliferation(P<0.01) and induced cell apoptosis,whereas bcl-2 and bax expression were not changed obviously in Hela cell treated by endostatin.Conclusion:Endostatin might have its antitumor effects on uterine cervix cell Hela and that at least in part.One of the important mechanisms of endostatin action on the potent antiangiogenic and antitumor activities may involve inducing apoptosis. |
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| Keywords: | bcl-2/bax |
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