MT1X基因多态性与2型糖尿病的关系

目的:探讨金属硫蛋白(MT)1X基因多态性与中国汉族人群中2型糖尿病（T2DM）发病的关系。方法:采用PCR技术和限制性片段长度多态性(RFLP)的方法检测132例糖尿病患者和137名正常对照者的MT基因家族中的MT1X基因（rs4531729）501T→C突变,统计各组对象的突变频率。结果:糖尿病组和正常对照组MT1X基因（rs4531729）501C→T突变型等位基因(C)频率差异无显著性 (χ²=1.508,P>0.05);两组间的CC、CT和TT 3种基因型频率分布差异无显著性(χ²=1.581,P>0.05)。结论: MT1X(rs4531729位点)突变型等位基因与T2DM的发生无关联性。

Association between metallothionein 1X gene polymorphism and type 2 diabetes mellitus

Objective To detect the association between metallothionein (MT) 1X gene polymorphism and type 2 diabetes mellitus (T2DM) in Chinese Han people. Methods The techniques of PCR and restriction fragment length polymorphism (RFLP) were used to examine MT4 gene (rs4531729) C→T mutation in 132 patients with T2DM and 137 unrelated healthy individuals, and then the mutation frequency was statistically computed. Results The alleic frequency (C) of MT 1X gene mutation was found no difference between the diabetics and non-diabetics (X2 = 1. 508, P>0. 05), and the distribution rates of CC, CT and TT also had no significant difference (X2 = 1.581, P>0. 05) between the case and the control. Conclusion There's no association between the alleic gene of MT 1X gene mutation and T2DM, and the mutation genotype may not increase the risk of suffering from T2DM.