系统性硬皮病抗核抗体谱的检测意义

目的探讨抗核抗体（ANA）和抗核抗体谱（ANAs）对系统性硬皮病的诊断意义。方法分别采用间接免疫荧光法（IIF）和免疫印迹法（IB）对64例SSc患者和30例健康对照者血清中6种自身抗体进行测定。结果IIF检测ANA结果显示，SSc患者中ANA的阳性率为98．4％（63／64），其荧光染色模型主要为均质核仁型；IB检测6种ENA结果显示，SSc患者以抗Scl-70抗体、抗SS-A抗体和抗nRNP抗体为主，阳性率分别为65．1％（41／64），29．7％（19／64）和6．3％（4／64）。结论多种自身抗体同时检出，可能提示SSc患者同时伴随有其他的自身免疫性疾病，或者有发生其他自身免疫性疾病的危险；此外，IB法对ANAs检测的阳性率和特异性均较高，对SSc的诊断帮助很大。

The clinical significance of antinuclear antibody repertoire detection in systemic sclerosis

Objective To evaluate the clinical significance of antinuclear antibody (ANA) and ANA repertoire in the diagnosis of systemic sclerosis (SSc). Methods ANAs were tested in 64 pa tients with SSc and 30 healthy controls by applying indirect immunofluorescence (IIF) and immunoblotting (IB). Results IIF-ANA assay showed that the positive rate of ANAs was 98. 4% (63/64) in SSc, and ANA staining was mainly shown as homogeneous-nucleolar pattern; IB-ENA test indicated that the antinuclear antibodies were mainly anti-Scl-70, anti-SS-A and anti-nRNP in SSc, and the positive rate was respectively 65.1% (41/64), 29.7% (19/64) and 6.3% (4/64). Conclusion Existence of multiple antibodies in SSc patients may hint that the patients might have other autoimmune diseases or increased risk for other autoimmune diseases. Besides, IB assay significantly increases the sensitivity and specificity of ANAs measurement, and contributes to the diagnosis of SSc.