Extraction,purification of prostate-specific antigen (PSA), and establishment of radioimmunoassay system as a diagnostic tool for prostate disorders |
| |
| Authors: | Ahmed Sami Abu-Bakr El-Bayoumy Akaber Tarek Hessien Keshta Khaled Mohamed Sallam Nahed Hassan Ebeid Hatem Mohamed Elsheikh Bsheer El-Sayed Bayoumy |
| |
| Institution: | 1. Department of Labeled Compounds, Hot labs Centre, Atomic Energy Authority, Cairo, Egypt;2. Chemistry Department, Faculty of Science, Zagazig University, Zagazig, Egypt |
| |
| Abstract: | This study aimed to provide an easy and effective method for extraction and purification of prostate-specific antigen (PSA) from human seminal fluid with high quantity (14 mg) and high purity (98%). The obtained PSA was injected into rabbits for production of anti-PSA polyclonal antibody (titer 1/1000), labeled with radioactive iodine-125 for preparation of radioactive PSA tracer (purity 98 ± 1.8% and specific activity 64 ± 1.9 µCi/µg), and used in preparation of PSA standards. All prepared components can be used in PSA immunoassays specially radioimmunoassay (RIA) kit preparation as a diagnostic tool for prostatic diseases. |
| |
| Keywords: | Prostate-specific antigen antigen purification immunization radio labeling radioimmunoassay |
|
|
