Atrial and brain natriuretic peptides enhance dopamine accumulation in cultured rat hypothalamic cells including dopaminergic neurons. |
| |
| Authors: | K Kadowaki K Hirota K Koike M Ohmichi A Miyake O Tanizawa |
| |
| Institution: | Department of Obstetrics and Gynecology, Osaka University Medical School, Japan. |
| |
| Abstract: | Dopamine accumulation in hypothalamic cells by atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) was analyzed using newborn rat hypothalamic cells in culture. Both ANP and BNP caused a dose-dependent increase in 3H]-dopamine accumulation in the cells. ANP increased 3H]-dopamine accumulation significantly within 20 min. The effects of ANP and BNP on dopamine accumulation paralleled an increase in intracellular cGMP concentration. (Bu)2-cGMP and sodium nitroprusside, a stimulator of the soluble form of guanylate cyclase, also enhanced 3H]-dopamine accumulation. ANP had no effect on efflux of 3H] radioactivity after 3H]-dopamine uptake. These results suggest that a change in cGMP is one of the intermediate steps in dopamine accumulation in hypothalamic cells by ANP and BNP. |
| |
| Keywords: | |
|
|
