十堰地区妇女宫颈人乳头瘤病毒感染及基因亚型分布情况调查分析

目的:对十堰地区妇女宫颈(HPV)亚型进行筛查,以探讨其分布规律。方法:采用核酸分子快速导流杂交基因芯片技术,对1 116例妇科就诊患者的宫颈刷片标本进行了21种HPV基因分型检测。结果:1 116例样本中,HPV感染者283例,总感染率25.36%,高危型HPV感染率14.52%,低危型HPV感染率2.96%,高危型及低危型混合感染阳性率7.89%;单一基因型别195例,占阳性患者的68.91%;双重混合感染59例,占阳性患者的20.85%;三重以上混合感染者29例,占阳性患者的10.25%。HPV感染阳性率居前5位的高危亚型依次是HPV16型50例(4.48%)、HPV52型47例(4.21%)、HPV53型28例(2.51%)、HPV58型17例(1.52%)及HPV39型15例(1.34%),常见的低危型是HPV6(1.26%)和HPV42(1.26%)。结论:十堰地区妇女HPV感染率较高,且以高危型HPV感染为主,感染型别既符合亚洲及中国地区人群的分布规律,又有独特的区域分布特点,并且多重感染率高,值得重视。

Distribution of cervical HPV infection and HPV gene subtypes of women in Shiyan area

Objective.. To screen subtypes of the cervical human papilloma virus （HPV） in women from Shiyan Region and explore their distributions. Method..Twenty-one HPV genotypes were detected from cervical cytology specimens of 1 116 gynecological patients by flow-through hybridisation and geno-typing technique （Hybri-Max）. Result;Of 1 116 subjects, there were 283 positive ones with HPV-infection rate of 25.36%. The infection rates of high-risk, low-risk and mixed were 14.52%, 2.96 % and 7.89%, respectively. Single genotype infection was ob- served in 195 of 283 （68.91%）, double in 59 （20.85%） and triple or multiple in 29 （10.25%）. The top five highrisk HPV subtypes in turn were HPV16 （50 cases, 4.48%）, HPV52 （47 cases, 4.21%）, HPV53 （28 cases, 2.51%）, HPV58 （17 cases, 1. 52%） and HPV39 （15 cases, 1.34%）. The common low-risk HPV subtypes were HPV6 （1.26%） and HPV42 （1.26 %）. Conclusion：Women from Shiyan Region had higher HPV-infection rate, mainly high-risk HPV-infection, which were accordance with the population distribution of Asia and China, and were characterized by unique regional distribution as well. Furthermore, it should be paid attention to high multiple infection rate.