分析147份输血前检查HBsAg呈弱阳性反应样本的复检结果

目的:通过对147份输血前检查乙型肝炎表面抗原(HBsAg)呈弱阳性反应样本的复检结果分析,总结用手工酶联免疫吸附试验(ELISA)法检测HBsAg过程中影响实验结果准确性的各种因素,为减小实验误差提供参考。方法:对147份HBsAg初次试验呈弱阳性反应的血清样本,由另一操作人员使用同一生产厂家的检测试剂进行双孔复检,复检结果S/CO值双孔均≥1者为阳性、S/CO值一孔≥1而另一孔1者为可疑、S/CO值双孔均1者为阴性。结果:147份HBsAg初次试验呈弱阳性的血清样本,双孔复检结果仍呈阳性反应者76份(51.7%)、可疑者5份(3.4%)、阴性者66份(44.9%),吸收度A值对比初次试验时下降者84份(57.1%)、升高者63份(42.9%),其中升高幅度最大者达16倍。结论:实验室工作人员不严格按试剂说明书操作是造成初次检测结果偏差较大的主要原因,对初次检测结果呈弱阳性反应的样本有必要进行双孔复检。对备血、输血和血液透析前的患者血样本,若能同检测供血者的血样本一样,采用由2位操作人员、2种检测试剂进行二次检测的模式,则更能保证结果的准确性。

Analysis of the retesting results for 147 samples with HBsAg weakly positive in the pre-transfusion test

Objective：By analyzingthe retesting results for 147 samples with Hepatitis B surface antigen（HBsag） weakly positive in the pre-transfusion test, to sum up various factors which affecting the accuracy of the experimental results during HBsAg process by hand enzyme linked immunosorbent assay（ELISA） method, and provide a reference for reducing experimental error. Method：The 147 serum samples with the initial test result of HBsAg weakly positive were double-hole retested by another operator using the same manufacturer＇s test reagent, retesting results of positive, suspicious and negative were S/CO value douhle-holes≥1, S/CO value a hole= 1 and the other a hole〈1, and S/CO value double hole 〈1. Result： Among the 147 serum samples with the initial test result of HBsAg weakly positive, there were 76 samples （51.7%） with positive results of double hole retest, 5 samples with suspicious （3.4%）, and 66 samples with negative （44.9%）. Compared to initial test, Absorbance A value of retesting results decline in 84 copies （57.1%）, and elevated in 63 copies （42.9%）, which the maximum amplitude increased up to 16 times . Conclusion： The main reason for large deviations of initial test results might be that laboratory staff did not operate strictly according to reagent instructions, and it would be necessary to double -holes retest samples with the initial test results of weakly positive. For the blood samples of preparation of blood, blood transfusion and before dialysis patients, if use the same testing mode with testing blood donors samples, namely two operators and two kinds of test reagent for secondary test, it could guarantee the accuracy of the results.