白细胞介素6与新生大鼠脑损伤的研究

目的　利用动物实验证实白细胞介素 6( IL-6)对未成熟脑的损伤作用 ,探讨 IL -6导致脑损伤的可能机制。　方法　在新生大鼠静脉内及侧脑室内注射不同剂量的重组人 IL-6( rh IL-6) ,分别于注射后 2 4及 72 h后处死 ,观察脑组织的病理变化。　结果　静脉注射 rh IL-61 0 0 0、50 0 0和1 0 0 0 0 U后 2 4 h,新生鼠脑组织表现为血管周围水肿及神经细胞的缺血性变 ,不同剂量 rh IL-6所致病变程度无明显差异。 72 h后血管周围水肿未见消退 ,神经细胞的缺血性变进一步发展为均质性变。侧脑室内注射 rh IL-61 0 0 0 U后 2 4 h脑组织病变与静脉注射后 2 4 h的病理表现基本相同 ;而注射rh IL-650 0 0 U后 2 4 h,蛛网膜下腔出血较静脉注射组明显。侧脑室内注射 72 h后 ,除有上述表现外 ,还出现了局部的脱髓鞘改变。　结论　外源性 IL-6对新生大鼠脑有不同程度的损伤作用。

The Relationship Detween Interleukin-6 and Drain Bamage in the Neonatal Rats

Objective To test whether IL 6 has any harmful effects on developing brain in neonatal rats, and try to illustrate its probable pathogenesis. Methods The neonatal rats were injected with different doses of rhIL 6 intravenously or intraventricularly. Animals were killed at 24 or 48 hours after injection to observe the pathological changes of brain tissues. Results Among animals killed at 24 hours after the intravenous injection with 1 000 U or 5 000 U rhIL 6, perivascular edema and ischaemic changes of neurons appear in their brain. There is no difference in the pathological changes in the brain of the rats treated with different doses. 72 hours after the intravenous injection, edema is still obvious, and ischaemic cell change in neurons aggravates into homogenizing cell change. When the brains are examined at 24 hours after intraventricular injection with 1 000 U rhIL 6, the pathological changes are the same as those treated by intravenous injection. Subarachnoid hemorrhage occurs more frequently in animals examined at 24 hours after 5 000 U rhIL 6 intraventricular injection than in those with intravenous injection. Besides, there appears local demyelination in the brain examined at 72 hours after the intraventricular injection of 5 000 u rhIL 6. Conclusion IL 6 by intravenous injection and intraventricular injection has harmful effects on the brain of neonatal rats.