| siRNA沉默Smad3对人KFB细胞增殖、凋亡及合成MMP3的影响 |
| |
| 引用本文： | 刘波,张恒术,果磊.siRNA沉默Smad3对人KFB细胞增殖、凋亡及合成MMP3的影响[J].免疫学杂志,2009,25(3). |
| |
| 作者姓名： | 刘波 张恒术 果磊 |
| |
| 作者单位： | 重庆医科大学第一附属医院烧伤与整形美容外科a临床检验诊断学省部共建教育部重点实验室重庆市重点实验室
|
| |
| 基金项目： | 重庆医科大学创新基金 |
| |
| 摘 要： | 目的 研究siRNA特异性沉默Smad3基因对人瘢痕疙瘩成纤维细胞(keloid fibroblast,KFB)增殖、凋亡及合成基质金属蛋白酶3(matrix metalloproteinase3,MMP3)的影响.方法 设计合成3对Smad3特异性siRNA片段(Smad3-siRNA-Y1,Y2,Y3)和1对无关干扰片段,转染人KFB细胞,RT-PCR和Western blot方法检测Smad3-siRNA片段对Smad3基因的特异性沉默效果,MTT法检测其对细胞增殖的影响,流式细胞仪检测细胞周期及细胞凋亡的变化,免疫细胞化学法检测MMP-3表达量的变化.结果 75 nmol/L Smad3-siRNA-Y1处理KFB细胞48小时后可特异并有效地抑制Smad3基因的表达;抑制细胞增殖,使S期细胞减少,G0/G1幅期细胞增多;凋亡指数增加;细胞表达MMP-3增加.结论 Smad3-siRNA可特异性抑制KFB细胞中Smad3基因表达,明显抑制细胞增殖,诱导细胞凋亡,并加快其合成MMP3.
|
| 关 键 词： | RNA干扰 瘢痕疙瘩 成纤维细胞 |
Effects of SiRNA-mediated Smad3 silence on proliferation,apoptosis,and MMP3 synthesis in human keloid fibroblasts |
| |
| LIU Bo,ZHANG Hengshu,GUO Lei State key laboratory of Laboratory Medical Diagnostics,Ministry of Education.Effects of SiRNA-mediated Smad3 silence on proliferation,apoptosis,and MMP3 synthesis in human keloid fibroblasts[J].Immunological Journal,2009,25(3). |
| |
| Authors: | LIU Bo ZHANG Hengshu GUO Lei State key laboratory of Laboratory Medical Diagnostics Ministry of Education |
| |
| Institution: | LIU Bo,ZHANG Hengshu,GUO Lei State key laboratory of Laboratory Medical Diagnostics,Ministry of Education,Department of Burn , Plastic Surgery,First Affiliated Hospital,Chongqing Medical University,Chongqing 400016,China |
| |
| Abstract: | Objective To investigate the effects of siRNA-induced specific silence of Smad3 on the proliferation,apoptosis,and matrix metalloproteinase 3 (MMP3) synthesis in human keloid fibroblasts (KFB).Methods Three Smad3 specific siRNA fragments (Smad3-siRNA-Y1,Y2,and Y3) and one irrelevant siRNA fragment were designed,synthesized,and transfected into human KFB cells. RT-PCR and Western blot were employed to validate the specific silencing effect of Smad3-siRNA on Smad3 gene. MTT assay was performed to detect KFB c... |
| |
| Keywords: | Smad3 |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
