香附炮制前后挥发油的GC-MS指纹图谱对比研究

目的 建立香附生品、参照品和醋制品的挥发油GC-MS指纹图谱，拟从整体角度表征香附炮制前后化学信息的变化。方法 采用GC-MS法，建立了3组香附挥发油的指纹图谱，并采用中药色谱指纹图谱相似度评价软件A版进行数据分析。结果 香附各制品相似度良好，验证了前期筛选的炮制工艺稳定可行。得到了香附生品共有峰52个、参照品28个、醋制品53个，鉴定了其中46个峰。对比分析发现，香附炮制后其挥发油成分种类、质量分数均有所变化；以生品为参照，对比参照品和醋制品共有成分，结果醋制品挥发油成分种类保留更为完整。结论 以醋作为炮制辅料科学合理，可起到稳定保护香附挥发油成分的作用，为揭示香附醋制机制及其物质基础提供一定科学依据。

Comparative study on GC-MS fingerprints of volatile oil in crude and processed Cyperi Rhizoma

Objective To establish the GC-MS fingerprints of volatile oil in crude, water-processed, and vinegar-processed Cyperi Rhizoma, and to capture the whole scope of the chemical constituents changing. Methods The fingerprints of volatile oil in crude, water-processed, and vinegar-processed Cyperi Rhizoma were established by GC-MS. And the data were analyzed by chromatographic fingerprint similarity evaluation software for Chinese materia medica (Version A). Results The similarities of each group were good, which indicated that the technology was stable and feasible. The crude group gained 52 common peaks, 28 as the water-processed group, and 53 as the vinegar-processed group. Meanwhile, 46 chemical components among the peaks above were identified in total. Comparing with the three groups, the kinds and contents of many components in each group were changed after processing. With the crude group as reference, the differences between the water-processed group and vinegar-processed group were contrastively analyzed before and after processing, and the Cyperi Rhizoma processed with vinegar could remain the chemical constitutions in its volatile oil better. Conclusion Using vinegar as processing accessory is scientific and reasonable. Vinegar could play a role in protecting the compounds of volatile oil in Cyperi Rhizoma steadily.