蜡块的不同修片方式对病理制片质量的影响

目的探讨蜡块的不同修片方式对病理制片质量的影响,以寻求提高病理制片质量的方法。方法手术切除的人体标本经脱水、浸蜡后包埋成蜡块,随机分成两组,一组修片后经冰水混合物预处理再切片;另一组经冰水混合物预处理后再修片、切片。常规HE染色,光学显微镜观察。结果修片后预处理组蜡块切出的切片组织完整、厚薄均匀,无重叠、皱褶、脱片、空洞和龟裂,或波浪样改变,与预处理后修片组蜡块制作的切片有明显差异。结论蜡块在预处理前修片的制片效果要优于预处理后修片的制片效果,预处理前修片的制片方法是可行的,可明显提高病理制片质量。

Affection of Different Paraffin Slice Trimming on the Quality of Pathological Slice Making

Objective To discuss the affection of different paraffin slice trimming on the quality of pathological slice making. Methods Human body specimen from surgery were first dehydrated and wax - infused, and then embedded into wax blocks; they were randomly divided into 2 groups：GroupAand Group B; slice trimming was made to the wax blocks in GroupA before the pretreatment of ice water and then the slices were cut ; the wax blocks in Group B were first pretreated by ice water and then slice trimming and slice cutting were done; routine HE staining was made to all slices in both groups and observation was made under light microscope. Results The slices in Group A were of intact appear- ance and well -proportioned thickness without superposition, rumpling, unfailing, voids and crack, or waving chan- ges, they were obviously different from those in Group B. Conclusions Slice trimming before pretreatment can have a better slice making effect than slice trimming after pretreatment, and can obviously raise the quality of pathologic slices.