蔗糖环境对寡发酵链球菌与变异链球菌双菌种生物膜形成的影响

目的探讨蔗糖环境对寡发酵链球菌与变异链球菌双菌种生物膜形成的影响,并与血链球菌和变异链球菌的双菌种生物膜形成进行比较。方法运用菌落计数法观察蔗糖环境下唾液包被的玻璃生物模型中单/双菌株寡发酵链球菌,变异链球菌和血链球菌24 h生物膜形成情况;运用激光共聚焦显微镜观察蔗糖环境下寡发酵链球菌,变异链球菌和血链球菌单/双菌株生物模型中24h生物膜形成厚度。结果无糖环境下,单菌株模型中,菌落数:血链球菌(55.67±5.36)>变异链球菌(53.48±2.63)(P>0.05)>寡发酵链球菌(46.24±2.34)(P<0.05);生物膜厚度:血链球菌(17.23±3.82)>变异链球菌(15.16±4.21)(P>0.05)>寡发酵链球菌(10.54±4.37)(P<0.05)。双菌株模型中,变异链球菌菌落数降低幅度:血链球菌组>寡发酵链球菌组(P<0.05)。生物膜厚度:血链球菌组(8.12±2.82)<寡发酵链球菌组(11.27±3.55)(P<0.05)。蔗糖环境下,单菌株模型中,菌落数:变异链球菌(58.54±2.74)>血链球菌(51.87±5.35)>寡发酵链球菌(48.57±3.05)(P<0.05)生物膜厚度:变异链球菌(20.63±5.71)>血链球菌(13.37±4.93)>寡发酵链球菌(12.45±4.62)(P<0.05)双菌株模型中;变异链球菌菌落数降低幅度,寡发酵链球菌组>血链球菌组(P<0.05),生物膜厚度:寡发酵链球菌组(6.67±2.19)<血链球菌组(10.45±2.72)(P<0.05)。结论外界糖环境影响寡发酵链球菌和血链球菌对变异链球菌的抑制作用,蔗糖环境下,寡发酵链球菌的抑制作用强于血链球菌。

The influence of sucrose environment on the two-species biofilm formed by streptococcus oligofermentans and Streptococcus mutans

Objective To investigate the effect of sucrose environment on the biofilm formation of Streptococcus oligofermentans and Streptococcus mutans and was compare with the case of Streptococcus sanguis and Streptococcus mutans. Methods Colony counting method was used to observe the 24 hours formation of biofilms of single/double strains of Streptococcus oligofermentans, Streptococcus mutans and Streptococcus sanguis on the sucrose-coated glass in the biological model in sucrose environment. Laser scanning confocal microscope was used to observe the thickness of biofilm formation of single/double strains of Streptococcus oligofermentans, Streptococcus mutans and Streptococcus sanguis in the sucrose environment. Results In single-strain model in a sugar-free environment, the number of colonies is: Streptococcus sanguis(55.67 ±5.36) >Streptococcus mutans(53.48 ±2.63)(P >0.05) > Streptococcus oligofermentans(46.24 ± 2.34)(P <0.05);The thickness of biofilm is: Streptococcus sanguis(17.23 ±3.82) >Streptococcus mutans(15.16 ± 4.21)(P>0.05)> Streptococcus oligofermentans(10.54 ± 4.37)(P<0.05). In double strain model, the reduced number of Streptococcus mutans colonies is: Streptococcus sanguis > Streptococcus oligofermentans(P <0.05);The thickness of biofilm is: Streptococcus sanguis(8.12 ±2.82) < Streptococcus oligofermentans(11.27 ± 3.55)(P<0.05). In Single-strain model in the sucrose environment, the number of colonies is:Streptococcus mutans(58.54 ±2.74) > Streptococcus sanguis(51.87 ±5.35) > Streptococcus oligofermentans(48.57 ±3.05)(P<0.05);The thickness of biofilm is: Streptococcus mutans(20.63±5.71)> Streptococcus sanguis(13.37±4.93)> Streptococcus oligofermentans(12.45 ± 4.62)(P<0.05). In double strain model, the reduced number of Streptococcus mutans colonies is: Streptococcus oligofermentans >Streptococcus sanguis(P <0.05);The thickness of biofilm is:Streptococcus oligofermentans(6.67 ± 2.19)< Streptococcus sanguis(10.45 ± 2.72)(P<0.05). Conclusion The sugar environment affects the inhibition of Streptococcus oligofermentans and Streptococcus sanguis on Streptococcus mutans.The inhibition of Streptococcus oligofermentans is stronger than Streptococcus sanguis in the sucrose environment.