Stromal vascular fraction improves deep partial thickness burn wound healing |
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| Affiliation: | 1. Private Medisu Hospital, Department of Plastic Reconstructive and Aesthetic Surgery, Antalya, Turkey;2. Erciyes University Medical Faculty, Department of Plastic Aesthetic and Reconstructive Surgery , Kayseri, Turkey;3. Erciyes University Medical Faculty, Department of Pathology, Kayseri, Turkey;1. Department of Life Sciences, Glasgow Caledonian University, Glasgow G4 0BA, UK;2. Scottish MRSA Reference Laboratory, Stobhill Hospital, 133 Balornock Road, Glasgow, UK;3. MRC/UCL Centre for Medical Molecular Virology and Wohl Virion Centre, Division of Infection & Immunity, UCL, London, UK;4. CEITEC – Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic;1. Health Science Center, Londrina State University, Londrina, Paraná, Brazil;2. Department of Chemistry, Londrina State University, Londrina, Paraná, Brazil;1. Department of Plastic Surgery, Breast Surgery and Burns, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark;2. Cell Therapy Facility, The Blood Bank, Department of Clinical Immunology, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark;3. Cardiology Stem Cell Laboratory, The Heart Centre, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark;4. Department of Radiology, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark;5. Department of Pathology, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark;6. Department of Clinical Genetics, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark;1. Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA, USA;2. Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA;3. McGowan Institute for Regenerative Medicine, Pittsburgh, PA, USA;1. Cytori Therapeutics Inc, San Diego, CA, USA;2. Comparative Biosciences Inc., Sunnyvale, CA, USA;3. UCSD Medical Center, University of California, San Diego, CA, USA |
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| Abstract: | ObjectiveThe practice of early burn wound excision and wound closure by immediate autologous skin or skin substitutes is the preferred treatment in extensive deep partial and full-thickness burns. To date there is no proven definite medical treatment to decrease burn wound size and accelerate burn wound healing in modern clinical practice. Stromal vascular fraction is an autologous mixture that has multiple proven beneficial effects on different kinds of wounds. In our study, we investigated the effects of stromal vascular fraction on deep partial-thickness burn wound healing.MethodsIn this study, 20 Wistar albino rats were used. Inguinal adipose tissue of the rats was surgically removed and stromal vascular fraction was isolated. Thereafter, deep second-degree burns were performed on the back of the rats by hot water. The rats were divided into two groups in a randomized fashion. The therapy group received stromal vascular fraction, whereas the control group received only physiologic serum by intradermal injection. Assessment of the burn wound healing between the groups was carried out by histopathologic and immuno-histochemical data.ResultsStromal vascular fraction increased vascular endothelial growth factor, proliferating cell nuclear antigen index, and reduced inflammation of the burn wound. Furthermore, vascularization and fibroblastic activity were achieved earlier and observed to be at higher levels in the stromal vascular fraction group.ConclusionsStromal vascular fraction improves burn wound healing by increasing cell proliferation and vascularization, reducing inflammation, and increasing fibroblastic activity. |
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| Keywords: | Deep second degree burn Deep partial thickness burn Burn wound Stromal vascular fraction Adipose tissue derived stem cell Burn wound healing stem cell |
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