| 体外负压培养对hBMSCs成骨活性影响的初步研究 |
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| 引用本文： | 朱养均,杨治,何鹏,张银刚.体外负压培养对hBMSCs成骨活性影响的初步研究[J].中国矫形外科杂志,2011,19(9):765-768. |
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| 作者姓名： | 朱养均 杨治 何鹏 张银刚 |
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| 作者单位： | 1. 陕西中医学院附属西电医院骨科,西安,710077
2. 西安交通大学医学院第一附属医院骨科,西安,710061 |
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| 基金项目： | 国家自然科学基金资助项目(编号:30973063); 西安市卫生局科技立项(编号:J2010B31); 陕西省卫生厅科研基金项目(编号:2010D23) |
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| 摘 要： | 目的]探讨体外负压培养对hBMSCs成骨活性的影响.方法]取第3代hBMSCs分为实验组和对照组,实验组进行间歇性负压培养,设置压力为17 kPa,30 min/次,4次/d,干预2周;对照组于普通CO,培养箱中常规培养.倒置显微镜下观察细胞形态,MTT法和流式细胞仪检测细胞增殖和凋亡,检测ALP活性,茜素红染色观察钙节结形成,免疫组织化学检测Ⅰ型胶原和VEGF的表达.结果]诱导2周,实验组细胞增殖能力下降,S期细胞百分比为(5.14±1.56)%,较对照组(13.45±3.51)%约下降62.4%,细胞凋亡增加.实验组ALP活性为(15.68±1.97)mU/mg,对照组为(6.344±1.21)mU/mg,两组比较差异有统计学意义(P<0.05);与对照组比较,实验组钙结节形成增多,VEGF表达较对照组显著提高;实验组Ⅰ型胶原表达呈阳性,对照组呈阴性反应.结论]负压能抑制hBMSCs增殖,促进细胞凋亡,但可以提高细胞成骨活性.
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| 关 键 词： | 负压 hBMSCs 成骨 |
Effects of negative pressure on osteogenesis in human BMSCs cultured in vitro |
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| ZHU Yang-Jun,YANG Zhi,HE Peng,et al..Effects of negative pressure on osteogenesis in human BMSCs cultured in vitro[J].The Orthopedic Journal of China,2011,19(9):765-768. |
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| Authors: | ZHU Yang-Jun YANG Zhi HE Peng |
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| Institution: | ZHU Yang-Jun,YANG Zhi,HE Peng,et al.Department of Orthopaedics,the Xidian Affiliated Hospital,Shanxi Traditional Chinese Medicine College,Xi' an 710077,China |
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| Abstract: | Objective]To investigate the effects of negative pressure on osteogenesis in human bone marrow stem cells(hBMSCs) cultured in vitro.Methods]The third passage hBMSCs were divided into negative pressure treatment group and control group.The treatment group was induced by negative pressure intermittently(pressure:17 kPa,30 min/at a time,4/times a day).The control group was cultured in conventional condition.The differentiation of hBMSCs was examined by invert phase contrast microscopy,the determination of al... |
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| Keywords: | negative pressure human bone marrow stem cells osteogenesis |
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