降糖通络颗粒HPLC指纹图谱研究

目的建立降糖通络颗粒HPLC指纹图谱，为科学地评价降糖通络颗粒的质量提供依据。方法样品测定采用AgilentZORBAXEclipseXDB—C18色谱柱（250mm×4．6mm，5μm），以乙腈-0．2％磷酸水为流动相进行梯度洗脱，采集230nm特征吸收波长下的信号，记录10批样品的HPLC特征图谱；采用“中药色谱指纹图谱超信息特征数字化评价系统”对图谱进行分析。结果以丹酚酸B为参照物，10批样品所得特征图谱共标定18个共有峰，相似度均在0．93以上。方法的精密度、稳定性和重复性良好。结论本试验建立的方法克服了单含量测定时信息量有限的缺点，能较全面、真实地揭示中药材的内在质量特征，可为降糖通络颗粒的质量控制和评价提供依据。

HPLC Fingerprint of Jiangtang Tongluo Granule

Objective To establish the HPLC fingerprint analysis for the quality control ofJiangtang Tongluo Granule. Methods The HPLC analysis was performed on an Agilent ZORBAX Eclipse XDB-C18 column （250 mm×4.6mm,5μm）; Acetonitrile-0.2% orthophosphoric acid aqueous solution was used as the mobile phase eluted gradiently; UV-detector was used to collect the signals at 230 nm wavelength. The HPLC characteristic chromatograms of ten batches of samples were determined. The characteristic chromatograms were comprehensively evaluated by the Digitized Evaluation System of the Chinese Materia Medica Fingerprint With the Super-information Characteristics. Results Eighteen common peaks were identified in the HPLC characteristic chromatograms of ten batches ofJiangtang Tongluo Granule by using the peak of salvianolic acid B as referential peak. The similarity of the ten batches of samples was over 0.93. The methodological validation results were good. Conclusion The fingerprint could effectively control the quality of Jiangtang Tongluo Granule, overcome the limited information of single content determination, and offer the guarantee for revealing the characteristics of traditional Chinese medicinal materials, which could provide the evidence for the identification and quality control of Jiangtang Tongluo Granule.