山茱萸总甙对大鼠角膜移植排斥反应免疫抑制作用机制研究

目的：观察山茱萸总甙对大鼠角膜移植模型细胞免疫功能影响，并通过观察外周血淋巴细胞活化状态以期进一步阐明山茱萸总甙的免疫抑制机制。方法： 建立封闭群大鼠SD-Wistar组间同种异体穿透性角膜移植模型，观察脾淋巴细胞对丝裂原刀豆蛋白A(ConA)刺激的增殖反应和单向混合淋巴细胞反应；采用双标法流式分析技术(PE标记CD4单抗及FITC标记的CD8、CD25单抗)，观察角膜移植术后不同时间外周血淋巴细胞表型变化，并计算CD4/CD8比值。 结果： ①山茱萸总甙可以明显抑制同种异体穿透性角膜移植大鼠T淋巴细胞的增殖反应和单向混合淋巴细胞反应。②外周血淋巴细胞表型改变主要结果如下：空白对照组大鼠外周血CD4、CD8的表达及CD4/CD8比值在术后各时点其差别无显著。但术后表达CD25的CD4阳性细胞明显升高。治疗组CD4/CD8比值则下降，术后表达CD25的CD4阳性细胞明显低于对照组。 结论： 抑制T淋巴细胞的增殖、混合淋巴细胞反应、T淋巴细胞活化表达CD分子，IL-2受体的表达，可能是山茱萸总甙抑制机体细胞免疫功能的主要机制之一。

Immunosupressive mechanism of cornus officinalis glycosides on the corneal allograft rejection in rats

AIM: To investigate the influence of the cornus officinalis glycosides (COG) on immunological function of corneal transplantation model of rats, and to clarify the immunosuppressive mechanism of COG through observing the activation of lymphocytes in blood. METHODS: Wister rats were used as recipients and SD rats were used as corneal graft donors, then the corneal allografts transplantation model on the closed colony rats were set up. Splenocytes proliferation and mixed lymphocyte reaction of Wister rats activated by ConA were observed. The phenotype change of CD4, CD8, CD25 in blood in different time postoperatively were observed by the di-sign flow cytometry, and the rate of CD4/CD8 was calculated. RESULTS: 1. The COG suppressed the proliferation of T lymphocytes and one-way mixed lymphocyte reaction on the corneal allografting. 2. The phenotype change of lymphocytes in boold was as follows: there was no significant difference between the different time of the CD4, CD8 expression and the CD4/CD8 rate in blood of the control group. The CD4 positive cells expressed CD25 postoperatively increased obviously. The CD4/CD8 rate of medicine group had the tendency to decrease. The CD4 positive cells expressed CD25 postoperation in the medicine group were less than that in the control group obviously. CONCLUSION: The suppression of the T lymphocyte proliferation, mixed lymphocyte reaction, CD molecule expressed by the activated T lymphocytes and the IL-2 receptor expression may be the main immunosuppressive mechanisms of Cornus officinalis glycosides on the cell-mediated immunity.