Restoration of Mineral Depositions by Dexamethasone in the Matrix of Nonmineralizing Osteoblastic Cells Subcloned from MC3T3-E1 Cells |
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Authors: | T T Baba |
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Institution: | (1) Department of Oral Biochemistry, School of Dentistry, Nagasaki University, 1-7-1 Sakamoto, Nagasaki 852-8588, Japan, JP |
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Abstract: | The original osteoblastic cell line, MC3T3-E1, was derived from normal mouse bone tissue and mineralized without any specific
factors in vitro. This cell line may be slightly unstable because of high differentiation, and some of these cells sometimes lost the ability
for mineral deposition. In this study, a new cell line was cloned which lost the ability for mineral deposition from MC3T3-E1
cells. This cell line, termed MC3T3-NM4, was not observed to undergo mineral deposition for up to at least 36 days even in
media containing beta-glycerophosphate. The alkaline phosphatase (ALP) activity was also not increased. The lack of calcifying
ability was found to be restored by the addition of dexamethasone in the media. This restoration was accompanied by an increase
in ALP activity and osteocalcin level. It was suggested that this restoration was not due to artificial mineralization resulting
from cell death.
Received: 3 March 1999 / Accepted: 25 May 2000 / Online publication: 22 September 2000 |
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Keywords: | : Dexamethasone — Glucocorticoid — Osteoblastic cell — MC3T3-E1 — MC3T3-NM4 |
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