特发性血小板减少性紫癜的B淋巴细胞的细胞周期分布

目的:探讨特发性血小板减少性紫癜(ITP)的B淋巴细胞的细胞周期分布和ITP的关系。方法:用碘化丙啶DNA染色法染色后用流式细胞仪检测40例初发的ITP患者治疗前、治疗恢复正常后及20例正常人的B淋巴细胞的细胞周期的分布比例,比较初发ITP患者治疗前、治疗恢复正常后与正常人的细胞周期分布的差异。结果:初发ITP组G0/G1期B淋巴细胞比例是(81.70±5.53)%,较治疗后恢复正常(90.38±0.92)%和对照组(91.53±2.09)少,差异有显著性(P<0.05);初发ITP组S期B淋巴细胞比例是(10.23±4.11)%,较治疗后恢复正常(5.60±0.91)%和对照组(5.18±1.21)%高,差异有显著性(P<0.05);初发ITP组G2/M期B淋巴细胞比例是(8.33±3.43)%,较治疗后恢复正常(4.03±1.76)%和对照组(3.30±1.84)%高,差异有显著性(P<0.05)。治疗后恢复正常和正常组比较G0/G1期、S期、G2/M期B淋巴细胞无差异(P>0.05)。结论:初发ITP患者治疗前S期和G2/M期B淋巴细胞比例增加,提示ITP患者的B淋巴细胞有过度增殖的现象,抑制ITP患者B淋巴细胞的细胞周期的转换可能是治疗ITP的新的治疗途径。

Cell Cycle Distribution of B Lymphocytes in Patients with Idiopathic Thrombocytopenic Purpura

Objective: To explore the relationship between idiopathic thrombocytopenic purpura(ITP) and cell cycle distribution of B lymphocytes.Methods: DNA of B lymphocytes extracted from 40 patients with ITP before and after treatment and from 20 normal subjects was stained with propidium iodide,and the cell cycle distribution was analyzed by flow cytometry.Results: The percentage of B lymphocytes in G_(0)/G_(1) phase(81.7±5.53)% was significantly lower than in the control group((91.53)±2.09)% and ITP group after treatment(90.38±0.92)%;the percentages of B lymphocytes in the S and G_(2)/M phase(10.23±4.11 and 8.33±3.43,respectively) were significantly higher than those in the control group(5.18±1.21 and 3.3±1.84,respectively) and ITP group after treatment(5.6±0.91 and 4.03±1.76,respectively).Conclusion: The proportions of S and G_(2)/M phase of B lymphocytes before treatment were increased,which indicated that the B lymphocytes of the patients attacked by ITP had over-proliferated.It may be a new method to inhibit the transition of B lymphocyte cell cycle in ITP patients.