| 高效液相色谱法测定肝积颗粒中芍药苷的含量 |
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| 引用本文： | 梁鹦,韩晓萍.高效液相色谱法测定肝积颗粒中芍药苷的含量[J].辽宁中医学院学报,2010(10):184-185. |
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| 作者姓名： | 梁鹦 韩晓萍 |
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| 作者单位： | [1]青海省中医院,青海西宁810000 [2]青海省药品检验所,青海西宁810000 |
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| 摘 要： | 目的：建立肝积颗粒中芍药苷的含量测定方法。方法：采用高效液相色谱（HPLC）法,以十八烷基硅烷键合硅胶为填充剂;以乙腈：0.1%磷酸溶液（15：85）为流动相;检测波长为230nm;柱温：25℃。结果：芍药苷在0.4092～2.0460μg线性关系良好,r=0.99993,平均回收率98.3%,RSD=0.5%（n=6）。结论：所用方法简便,准确度高,重现性好,可用于肝积颗粒的质量控制。
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| 关 键 词： | 肝积颗粒 质量标准 高效液相色谱 |
Evaluating Peoniflorin in Gan-Ji Granules with High Performance Liquid Chromatogram |
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| LIANG Ying,HAN Xiao-ping.Evaluating Peoniflorin in Gan-Ji Granules with High Performance Liquid Chromatogram[J].Journal of Liaoning College of Traditional Chinese Medicine,2010(10):184-185. |
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| Authors: | LIANG Ying HAN Xiao-ping |
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| Institution: | 1.Qinghai Province Hospital of Traditional Chinese Medicine,Xining 810000,Qinghai,China ;2.Qinghai Province Institute for Drug Control,Xining 810000,Qinghai,China) |
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| Abstract: | Objective：Find a method to evaluate peoniflorin in Gan-Ji Granules.Methods：Apply HPLC,octadecyl silane chemically bonded silica as loading agent,methyl cyanide：0.1% orthophosphoric acid（15：85）as moving phase,detection wavelength is 230nm and column temperature is 25℃.Results：Linear relationship is good when peoniflorin is 0.4092 ～2.0460μg,r=0.99993,average recovery is 98.3%,RSD=0.5%（n=6）.Conclusions：This method features easy use,high accuracy rating and nice reproducibility,so it is suitable to be applied in quality control of Gan-Ji Granules. |
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| Keywords: | Gan-Ji granules quality specification high performance liquid chromatogram |
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