类风湿关节炎患者HLA-DR4/1亚型与抗环瓜氨酸肽抗体的关系

目的]探讨类风湿关节炎（rheumatoid arthritis，RA）患者HLA—DR4／1分子与血清抗环瓜氨酸肽（cyclic citrullinated peptide，CCP）抗体之间的关系。方法]收集118例RA患者，以序列特异性引物聚合酶链反应（PCR—SSP）技术对上述患者进行HLA—DR4／1分型，酶联免疫吸附试验（ELISA）方法检测患者血清抗CCP抗体水平。结果]在118例RA患者中，35例患者HLA—DR4阳性，其中DRB1^＊0405阳性20例；5例患者HLA—DR1阳性。在118例RA患者中，抗CCP抗体阳性有90例。其中，在40例HLA—DR4／1 阳性的RA患者中，抗CCP抗体阳性的占82．5％（33／40）；在其余78例HIA—DR4／1阴性RA患者中，73．1％（57／78）抗CCP抗体阳性。HLA～DR4／1阳性患者与HLA—DR4／1阴性患者抗CCP抗体的阳性率差异无统计学意义（P=0．255）。而20例HLA—DRB1^＊0405阳性的RA患者抗CCP抗体阳性者19例，HLA—DRB1^＊0405阳性RA患者抗CCP抗体阳性率高于其他HLA—DR4／1亚型阳性的RA患者，也高于HLA—DR4／1阴性患者。结论]RA中HIJA—DRB1^＊0405基因与抗CCP抗体具有一定的相关性。

Relationship between HLA-DR4/1 and anti- cyclic citrullinated peptide antibody in rheumatoid arthritis

Objective] To explore the relationship between HLA-DR4/1 and anti-cyclic citrullinated peptide(CCP) antibody in rheumatoid arthritis(RA).Methods] Serum were obtained from 118 RA patients.HLA-DR4/1 subtypes were analyzed by PCR-SSP.Antibodies to CCP were determined by ELISA.Results] Of 118 RA patients,40 patients(33.9%) were positive for HLA-DR4/1 subtypes including 20 HLA-DRB1*0405 positive patients,and 78 patients(66.1%) were negative for HLA-DR4/1 subtypes.In HLA-DR4/1 positive group,anti-CCP antibodie...