家族史阳性和阴性食管癌患者癌组织中FHIT和BRCA2及MLH1的表达变化

目的:探讨FHIT、BRCA2及MLH1在河南食管癌高发区家族史阳、阴性食管癌患者癌组织中的表达变化,及其与食管癌遗传易感性之间的关系。方法:采用ABC法检测河南省食管癌高发区林州市74例食管癌患者(其中33例家族史阳性,41例家族史阴性)手术切除标本癌组织FHIT、BRCA2及MLH1蛋白表达情况。结果:FHIT56%(18/33)]、BRCA267%(22/33)]和MLH173%(24/33)]在家族史阳性食管癌患者癌组织中阴性表达率,显著高于家族史阴性食管癌患者27%(11/41)、37%(15/44)和27%(16/41)],P值均<0·05。结论:FHIT、BRCA2和MLH1蛋白可能是影响食管癌遗传易感性的重要分子事件之一。

Alterations of FHIT, BRCA2 and MLH1 expressions in esophageal cancer tissues from the esophageal cancer patients with and without family history

OBJECTIVE:To explore the alterations of FHIT,BRCA2 and MLH1 expressions in esophageal cancer (EC) tissues from the patients with and without EC familial history at a high-incidence area for EC in Henan Province, and to correlate these changes with EC hereditary susceptibility. METHODS:The immunohistochemical method (ABC) and histopathological method were applied to test 74 EC specimens and the expressions of FHIT,BRCA2 and MLH1 proteins in SCC were correlated with the hereditary susceptibility distribution. Thirty-three of the 74 EC specimens were from the families which had two or more EC patients in continuous two generations (positive EC family history, ECFH ) and 41 specimens were from the families without positive tumor history (ECFH-). RESULTS:The negative expression of FHIT gene was significantly higher in ECFH (56%,18/33) than that in ECFH-(27%,11/41, P< 0.05). The negative expression of BRCA2 gene was significantly higher in EC tissue from the ECFH than that from the ECFH-67%( 22/33 ) vs 37%(15/41),P<0.05). The negative expression rate for MLH1 in the ECFH (73%,24/33) was higher than that in the ECFH-(27%, 16/41 ), P< 0.01.CONCLUSION:FHIT,BRCA2 and MLH1 may play an important role in the EC familial hereditary susceptibility.