| CoCl_2对原代培养的猪肺动脉内皮细胞钙离子的影响 |
| |
| 引用本文： | 黄文峰,廖贵华.CoCl_2对原代培养的猪肺动脉内皮细胞钙离子的影响[J].中西医结合研究,2010,2(4):182-184. |
| |
| 作者姓名： | 黄文峰 廖贵华 |
| |
| 作者单位： | 1. 三峡大学医学院形态学部,湖北宜昌,443002
2. 宜昌市中医院中西医结合耳鼻喉科,湖北宜昌,443003 |
| |
| 摘 要： | 目的探讨氯化钴(CoCl2)对原代培养的猪肺动脉内皮细胞钙离子浓度的影响。方法原代培养猪肺动脉内皮细胞,分别用不同浓度的CoCl2刺激2min,用双波长荧光分光光度计观察细胞质内钙离子浓度的变化;另外,用1mmol/L的CoCl2刺激细胞不同时间,观察细胞质内钙离子浓度的变化。结果不同浓度的CoCl2对猪肺动脉内皮细胞内钙离子浓度无显著影响;1mmol/L的CoCl2刺激细胞不同时间也对细胞内钙离子浓度无显著影响。结论与物理缺氧方法不同,化学缺氧试剂CoCl2对原代培养的猪肺动脉内皮细胞的钙离子浓度没有明显影响,表明猪肺动脉内皮细胞对CoCl2的刺激有一定的耐受性。
|
| 关 键 词： | 内皮细胞 氯化钴 钙离子 |
Role of Cobalt Chloride on the Cytosolic Calcium Concentration of Primary Cultured Porcine Pulmonary Artery Endothelial Cells |
| |
| Authors: | HUANG Wenfeng LIAO Guihua |
| |
| Institution: | HUANG Wenfeng,LIAO Guihua1Department of Morphology,Medical College of Three Gorges University,Hubei,Yichang 443002,China2Department of Otolaryngology,Yichang Hospital of Chinese Medicine,Yichang 443003,China |
| |
| Abstract: | Objective To study the role of Cobalt Chloride (CoCl2) on the cytosolic calcium concentration of primary cultured porcine pulmonary artery endothelial cells.Methods The primary cultured porcine pulmonary artery endothelial cells were treated with different concentration of CoCl2 for 2 minutes,and the changes of cytosolic calcium concentration were observed by dual-wavelength fluorescence spectrophotometer.We also observed the changes of cytosolic calcium concentration after stimulating the cells with CoCl2 ... |
| |
| Keywords: | endothelial cells Cobalt Chloride calcium |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
