肿瘤坏死因子受体及其信号转导蛋白TRAF2在喉癌中的表达

目的：探讨肿瘤坏死因子受体(TNFR)和其转导蛋白TRAF2的表达及其与喉鳞状细胞癌生物学行为的关系。方法：利用免疫组织化学及原位末端标记法，对33例喉鳞状细胞癌组织TNFR1、TNFR2和TRAF2的表达及其凋亡指数进行检测。结果：①33例喉鳞状细胞癌中，21例TNFR1表达阳性（63.6%），2例TNFR2表达阳性（6.1%），两者表达染色阳性部位主要集中在细胞膜上，表达程度与喉鳞癌的生物学行为无关（P＞0.05）；②33例喉鳞状细胞癌中，23例TRAF2表达阳性（69.7%），其表达部位主要位于细胞浆中。TRAF2的表达程度与喉鳞癌的病理分级有关（P<0.05），与其他的生物学行为无关（P＞0.05）；③TRAF2的表达与TNFR1的表达具有明显的正相关（r=0.637，P<0.01）； ④TNFR1、TRAF2表达阳性组织的细胞凋亡指数（3.12±1.47及2.85±1.19）显著低于TNFR1、TRAF2表达阴性组织（4.28±1.34及5.12±0.81）（P<0.05及P<0.01）。结论：TNFR1信号转导过程中募集TRAF2增强肿瘤细胞抗凋亡能力，与肿瘤的分化和恶性程度有一定关系，缺乏表达TNFR2可能对喉癌的发生发展有一定作用。

Expressions of TNFR1, TNFR2 and TRAF2 in laryngeal squamous carcinoma

Objective To study the expressions of TNFR1,TRAF2 and TNFR2 in laryngeal squamous carcinoma and the relationship with the biological characteristics of laryngeal squamous carcinoma.Methods By using immunohistochemical method and TUNEL method,the expressions of TNFR1,TNFR2,TRAF2 and the apoptotic index were examined in 33 laryngeal squamous carcinoma tissues.Results In the laryngeal squamous carcinoma cells,the positive rate of TNFR1 was 63.6%,the positive rate of TNFR2 was 6.1%,the positive rate of TRAF2 was 69.7%.Neither TNFR1 nor TNFR2 was related to the biological characteristic of laryngeal squamous carcinoma(P>0.05),and TRAF2 was related to the tumor grade only(P<0.05).The expression of TNFR1 was strongly associated with the expression of TRAF2(P<0.01).The apoptotic indexes of the positive expression tissues of TNFR1 and TRAF2(3.12±1.47,2.85±1.19) were significantly lower than those of the negative expression tissues(4.28±1.34,5.12±0.81)(P<0.05 and P<0.01).Conclusion TRAF2 could be a new diagnostic index in laryngeal squamous carcinoma.The negative expression of TNFR2 may participate in the progress of laryngeal squamous carcinoma.