白细胞介素6基因-174G/C和-634C/G多态性与冠心病的相关性研究

目的探讨白细胞介素6(interleukin6,IL6)基因启动子上游174G/C和634C/G基因多态性,在冠心病患者和正常人群中的分布及与冠心病的相关性。方法应用聚合酶链反应限制性片断长度多态性技术,对汉族199例冠心病患者及189名正常人群,白细胞介素6基因174G/C、634C/G位点进行研究,同时结合血脂、脂蛋白和载脂蛋白水平,探讨两者之间的关系。结果正常人群和冠心病患者的174G等位基因频率均为0.99。174C等位基因频率均为0.01。634C等位基因频率在正常人群和冠心病患者分别为0.82和0.76,G等位基因频率分别为0.18和0.24,两者差异有统计学意义(P<0.05)。冠心病患者634GG基因型频率(0.08)明显高于对照组(0.02)(P<0.05)。结论白细胞介素6基因174位点多态性与冠心病无关,而634位点多态性与冠心病有相关性。G等位基因可能是汉族人群冠心病的易感性标志。

Relationship between coronary heart disease and polymorphisms of interleukin-6 gene

Objective To investigate the association between -174 G/C & -634C/G of interleukin-6 gene polymorphism and coronary heart disease in Chinese population.Methods The genotype and allele frequency of interleukin-6 gene polymorphisms was assayed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 199 patients with evident of CHD and 189 healthy individuals. The relation of gene polymorphisms of interleukin-6 and levels of serum lipids, lipoproteins, and apolipoproteins were also studied.Results The IL-6 -174 G allele was detected in 0.99 of normal controls and 0.99 of patients, while IL-6 -174 C allele was detected in 0.01of normal controls and 0.01 of patients. The IL-6( -634C) allele was detected in 0.82 of normal controls and 0.76 of patients, while IL-6 -634G allele was detected in 0.18 of normal controls and 0.24 of patients. The frequencies of GG genotype and IL-6 -634G allele were higher in coronary heart disease than in controls ( P< 0.05 ).Conclusion These results suggest that the interleukin-6 gene -174G/C polymorphism was not related to CHD. But -634C/G polymorphism was associated with CHD in Chinese population. The -634G allele might be a risk factor for CHD.