| 瘦素对GT1-7细胞GnRH释放的影响 |
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| 引用本文： | 杨颖,陈名道,李凤英,唐金凤,周丽斌,殷峻,李荣英. 瘦素对GT1-7细胞GnRH释放的影响[J]. 放射免疫学杂志, 2002, 15(2): 65-67 |
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| 作者姓名： | 杨颖 陈名道 李凤英 唐金凤 周丽斌 殷峻 李荣英 |
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| 作者单位： | 上海第二医科大学附属瑞金医院、上海市内分泌研究所,200025 |
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| 摘 要： | 目的：GT1－7细胞是替代研究GnRH神经元的理想细胞模型，本文观察瘦素（Leptin)对GT1－7细胞GnRH释放的影响。方法：采用氯胺T法放射性核素标记物^125I标记GnRH，建立了稳定灵敏的GnRH放射免疫分析方法。将不同浓度的瘦素，以不同的时间与GT1－7细胞孵育，用RIA法测定GT1－7细胞上清液中GnRH含量。结果：结果表明瘦素能直接作用于GT1－7细胞株，促进GnRH释放，以浓度为20ng/ml，孵育15分钟作用最明显。结论：瘦素可能通过直接作用于下丘脑GnRH神经元，而对生殖功能起调节作用。
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| 关 键 词： | 瘦素 GT1－7 细胞 促性腺激素释放激素 放射免疫分析 |
| 修稿时间： | 2002-02-28 |
The Effect of Leptin on GnRH Serection by GT1-7 Cell Line |
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| Yang Ying,Chen Mingdao,Li Fengyin,et al.. The Effect of Leptin on GnRH Serection by GT1-7 Cell Line[J]. Journal of Radioimmanology, 2002, 15(2): 65-67 |
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| Authors: | Yang Ying Chen Mingdao Li Fengyin et al. |
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| Abstract: | Objective To investigate the effects of leptin on GnRH serection from GT1-7 cell line, which is an ideal cell-madol for studying the function of GnRH neurons. Methods GT1-7 cells were treated with leptin at different concentrations for different periods, and GnRH was determined by RIA which was iodinated by chloramine T technique. Results Leptin induced a significant stimulation of GnRH release, the result being most significant at 20ng/ml for 15 minutes. Conclusion Leptin might participate in the regulation of reproductive processes by acting directly upon GnRH neurons. |
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| Keywords: | Leptin GT1-7 cell gonadotropin releasing hormone(GnRH) Radioimmunoassay(RIA) |
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