高温致神经管畸形中细胞增殖和细胞凋亡的定量研究

为探讨神经管畸形的发生机理,在高温致金黄地鼠神经管畸形的动物模型上,采用核固红-结晶紫染色方法和图像分析技术,对各期实验组和对照组鼠胚神经管及周围间充质细胞增殖与细胞凋亡的线参照数密度和面数密度进行了测量.结果显示,高温后8h神经管和周围间充质的细胞分裂比对照组明显减少,细胞凋亡明显增多,尤其是头部两侧程序性细胞死亡区域内的细胞凋亡增多显著.表明高温可抑制细胞增殖,促进程序性细胞死亡区域的细胞过度凋亡,导致神经管延迟闭合或不闭合,引起神经管畸形.

QUANTITATIVE STUDY OF CELL PROLIFERATION AND APOPTOSIS IN THE DEVELOPMENT OF NEURAL TUBE DEFECTS CAUSED BY HYPERTHERMIA

To explore the developmental mechanism of neural tube defects, using the animal experiment model with neural tube defects caused by hyperthermia, the nuclear fast red-crystal violet staining method and image analysis were used to quantitatively observe the cell proliferation and apoptosis in the embryonic neural epithelium and the mesenchyme around neural tube. The results showed that the number of mitotic cells of the neural epithellium and the mesenchyme around neural tube in the experimental groups decreased, the number of apoptotic cells increased when compared with that of the control groups at 8 and 16 hours after maternal hyperthermia, and cell death induced by hyperthermia in the lateral sides of head was always associated with the zones of the programmed cell death(PCD) seen in the control embryos. It indicated that hyperthermia may inhibit proliferation of neuroepithelium and the mesenchyme around neural tube, and induce excessive cell death in regions of PCD. It may be an important way by which hyperthermia causes neural tube defects.