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| 外源P16基因对人类恶性脑胶质瘤细胞系活性的影响 | |
| 引用本文： | 孙泽林,戚晓渊,孙泽辉,程爱国.外源P16基因对人类恶性脑胶质瘤细胞系活性的影响[J].华北煤炭医学院学报,2007,9(1):1-2. |
| 作者姓名： | 孙泽林 戚晓渊 孙泽辉 程爱国 |
| 作者单位： | 1. 华北煤炭医学院附属医院神经外科,河北唐山,063000 2. 华北煤炭医学院附属医院检验科,河北唐山,063000 3. 华北煤炭医学院附属医院临床医学系,河北唐山,063000 4. 河北省唐山市第三人民医院 |
| 基金项目： | 河北省自然基金资助项目。 |
| 摘 要： | ①目的探讨5型腺病毒载体（Ad5）携带P16基因对恶性脑胶质瘤细胞系T／905生长状态的影响。②方法免疲组织化学法测定P16蛋白表达；甲基噻唑基四唑法测定恶性脑腱质瘤细胞系生长状态及感染病毒细胞克隆形成数的测定。③结果重组体腺病毒能介导P16外源基因在恶性脑肢质瘤细胞系TJ905细胞中阳性表达，6天时肿瘤经胞生长抑制率为98％，并且能显著地抑制肿瘤细胞的克隆形成能力。④结论腺病毒介导P16基因能在肿瘤细胞中表达，并能明显抑制肿瘤细胞生长的状态。 |
| 关 键 词： | 腺病毒 脑胶质瘤 基因治疗 抑癌基因 P16 |
| 文章编号： | 1008-6633（2007）01-0001-02 |
| 收稿时间： | 2006-10-25 |
| 修稿时间： | 2006年10月25 |
Effects of exogenous P16 gene on activities of human glioma cell line |
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| SUN Zelin ,QI Xiaoyuan ,SUN Zehui,et al.Effects of exogenous P16 gene on activities of human glioma cell line[J].Journal of North China Coal Medical College,2007,9(1):1-2. | |
| Authors: | SUN Zelin QI Xiaoyuan SUN Zehui |
| Institution: | SUN Zelin ,QI Xiaoyuan ,SUN Zehui,et al(Department of Nervisurgiolog North China Medical College, Tangshan 063000, China) |
| Abstract: | Objective To study the effects of P16 gene taken by adenoviral vector(Ad5)on growth of human glioma cell line TJ905. Methods Expressions of P16 protein was detected in TJ905 cell line by immunohistochemical method. The proliferation and number of colony formation of the cell line was analyzed by MTT. Results The positive expressions of P16 in glioma cell line TJ905 was transmitted and colony formation of tumor cells was greatly inhibited by recombinant adenovirus, after 6 days the inhibitory rate of the growth of tumor cells was 98%. Conclusion Exogenous P16 mediated by adenovirus can be expressed in tumor cells and inhibited the growth of infected tumor cells. |
| Keywords: | Adenovirus Glioma Genetic therapy Anti-oncogene P16 |
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