| 高糖对人腹膜间皮细胞的增殖和损伤及分泌细胞因子的影响 |
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| 引用本文： | 刘映红,刘伏友,张浩,彭佑铭,袁芳,刘虹,成梅初,卓莉. 高糖对人腹膜间皮细胞的增殖和损伤及分泌细胞因子的影响[J]. 中南大学学报(医学版), 2006, 31(4): 575-579 |
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| 作者姓名： | 刘映红 刘伏友 张浩 彭佑铭 袁芳 刘虹 成梅初 卓莉 |
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| 作者单位： | 中南大学湘雅二医院肾内科,长沙,410011;湘雅三医院肾内科,长沙,410013 |
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| 摘 要： | 目的：探讨高糖介导腹膜纤维化的可能机制。方法：原代培养的第3代人腹膜间皮细胞（HPMCs）分为不同时间（24，48h）的对照组（F12）和高糖组（F12＋4％葡萄糖）。用MTT法测定细胞增殖，乳酸脱氢酶（LDH）法观察细胞损伤程度，采用酶联免疫法（ELISA）测定纤维连接蛋白（FN）、转化生长因子β1（TGF-β1）和结缔组织生长因子（CTGF）蛋白水平以及采用RT-PCR测定FN，TGF-β1和纤溶酶原激活物抑制剂-1（PAI-1）mRNA表达。结果：①高糖明显抑制细胞增殖，24h和48h高糖组与同一时间点对照组比较，MTT吸光度值均明显下降（P〈0．001或P〈0.01）；②高糖明显导致细胞损伤，24h和48h高糖组与同一时间点对照组比较，培养液中LDH含量均明显增加（均P〈0.001）；③高糖培养24，48h均使FN，CTGF和TGF-β1蛋白表达增加（P〈0．05或P〈0.001）；④高糖使FN，TGF-β1和PAI-1 mRNA表达均上调。结论：高糖能够抑制HPMCs增殖，损伤HPMCs，并刺激HPMCs分泌更多的TGF-β1，CTGF，FN和PAI-1，从而使HPMCs细胞外基质生成增多、降解减少，最终导致腹膜纤维化的形成。
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| 关 键 词： | 高糖 腹膜间皮细胞 增殖 损伤 细胞因子 |
| 文章编号： | 1672-7347（2006）04-0575-05 |
| 收稿时间： | 2006-05-10 |
| 修稿时间： | 2006-05-10 |
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| LIU Ying-hong,LIU Fu-you,ZHANG Hao,PENG You-ming,YUAN Fang,LIU Hong,CHEN Mei-chu,ZHUO Li.
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| Authors: | LIU Ying-hong LIU Fu-you ZHANG Hao PENG You-ming YUAN Fang LIU Hong CHEN Mei-chu ZHUO Li |
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| Affiliation: | .Department of Nephrology, Second Xiangya Hospital, Central South University, Changsha 410011, China. |
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| Abstract: | OBJECTIVE: To determine the mechanism of peritoneal fibrosis of peritoneal mesothelial cells by high glucose. METHODS: The third passage human peritoneal mesothelial cells (HPMCs) from primary culture were divided into a control group (F(12)) and high glucose groups (F(12)+4% glucose) in different times (24, 48 h). The cell proliferation was assayed by the method of MTT (methylthiazoletetrazolium). The cell damage was measured by LDH (lactate dehydrogenase). The protein expression of fibronectin (FN), transforming growth factor-beta1(TGF-beta(1)) and connective tissue growth factor (CTGF) were detected by ELISA. The mRNA expression of FN, TGF-beta(1) and PAI-1 were detected by RT-PCR. RESULTS: High glucose suppressed the cell proliferation. The result of MTT showed that compared with the control group, the value of OD of high glucose groups at 24 or 48 h decreased significantly (P<0.01 or 0.01); The cell damage was enhanced in high glucose groups, at 24 or 48 h compared with the control group at the same time (all P<0.01). The protein expressions of TGF-beta(1), CTGF and FN in supernate fluid of cell culture were significantly enhanced when high glucose stimulated the HPMCs in the high glucose groups at 24 or 48 h compared with the control group at the same time (P<0.05 or 0.001). The expressions of FN, TGF-beta(1) and PAI-1 mRNA were upregulated in 24 h high glucose group compared with that of 24 h control group. CONCLUSION: High glucose can suppress the HPMC proliferation and damage HPMCs. Increase of TGF-beta(1), CTGF, FN and PAI-1 of HPMCs stimulated by high glucose can promote the synthesis and decreased degradation of extracellular matrix, which might be related with the mechanism of peritoneal fibrosis of peritoneal mesothelial cells by high glucose. |
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| Keywords: | high glucose peritoneal mesothelial cells proliferation damage cytokines |
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