| 罗格列酮对2型糖尿病并发非酒精性脂肪肝大鼠肝脏解偶联蛋白-2表达的影响 |
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| 引用本文： | 陈玉华,武革,郭中秋,谭娅琴,李善清,陈晓铭.罗格列酮对2型糖尿病并发非酒精性脂肪肝大鼠肝脏解偶联蛋白-2表达的影响[J].胃肠病学和肝病学杂志,2011,20(2):113-117. |
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| 作者姓名： | 陈玉华 武革 郭中秋 谭娅琴 李善清 陈晓铭 |
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| 作者单位： | 1. 深圳市龙岗区人民医院内分泌科,深圳,518172
2. 广东医学院附属医院内分泌科 |
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| 基金项目： | 深圳市龙岗区科技计划医疗卫生项目(No.YL2009061) |
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| 摘 要： | 目的观察罗格列酮对2型糖尿病（T2DM）并发非酒精性脂肪肝（NAFLD）大鼠肝脏解偶联蛋白-2（UCP-2）表达的影响,探讨其防治T2DM并发NAFLD的部分作用机制。方法高脂高糖饮食结合小剂量链脲佐菌素（STZ）腹腔注射建立T2DM并发NAFLD大鼠模型,将成模大鼠随机分为模型组、罗格列酮治疗组,每组各16只,并设立正常对照组。治疗组给予罗格列酮3 mg/（kg.d）灌胃治疗。于实验第16（治疗后8周）、20周（治疗后12周）末分批处死大鼠,检测肝功能、空腹血糖、血脂和血清胰岛素水平,光镜下观察大鼠肝脏组织学形态,分别以免疫组织化学法和逆转录-聚合酶链反应（RT-PCR）法检测肝组织UCP-2蛋白和UCP-2mRNA的表达情况。结果与正常组相比,模型组大鼠血清转氨酶、空腹血糖、血清胰岛素及血脂水平均明显升高,胰岛素敏感指数明显下降（P均〈0.01）,肝脏出现不同程度脂肪变性,肝组织UCP-2蛋白和UCP-2 mRNA表达增高,以20周末最为明显。在16周末（治疗后8周）和20周末（治疗后12周）,治疗组大鼠血清转氨酶、血糖、血清胰岛素及血脂水平均有改善,胰岛素敏感指数明显升高,肝细胞脂肪变性明显减轻,肝组织UCP-2蛋白和UCP-2 mRNA表达明显下降,与模型组比较差异均有统计学意义（P〈0.01）。结论 T2DM并发NAFLD大鼠肝组织UCP-2表达增强,罗格列酮可以调控T2DM并发NAFLD大鼠肝脏UCP-2 mRNA和UCP-2蛋白的适度表达,这可能是其治疗T2DM并发NAFLD的分子机制之一。
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| 关 键 词： | 2型糖尿病 非酒精性脂肪肝 解偶联蛋白-2 罗格列酮 |
Effect of rosiglitazone on the expression of hepatic uncoupling protein 2 in type 2 diabetic rats complicated with non-alcoholic fatty liver disease |
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| CHEN Yuhua,WU Ge,GUO Zhongqiu,TAN Yaqin,LI Shanqing,CHEN Xiaoming.Effect of rosiglitazone on the expression of hepatic uncoupling protein 2 in type 2 diabetic rats complicated with non-alcoholic fatty liver disease[J].Chinese Journal of Gastroenterology and Hepatology,2011,20(2):113-117. |
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| Authors: | CHEN Yuhua WU Ge GUO Zhongqiu TAN Yaqin LI Shanqing CHEN Xiaoming |
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| Institution: | CHEN Yuhua1,WU Ge2,GUO Zhongqiu1,TAN Yaqin1,LI Shanqing1,CHEN Xiaoming2 1.Department of Endocrinology,People's Hospital of Longgang District,Shenzhen 518172,2.Department of Endocrinology,the Affiliated Hospital of Guangdong Medical College,China |
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| Abstract: | Objective To observe the effect of rosiglitazone on the expressions of uncoupling protein-2(UCP-2) in the liver of type 2 diabetic rats complicated with non-alcoholic fatty liver disease(NAFLD),and explore its mechanism of treating type 2 diabetes mellitus(T2DM) complicated with NAFLD.Methods The model rats of T2DM complicated with NAFLD were established by feeding high-glucose and high-fat diet,and injection of low dose streptozotocin.The model rats were randomly divided into 2 groups: model group(n=16) an... |
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| Keywords: | Type 2 diabetes mellitus Non-alcoholic fatty liver disease Uncoupling protein 2 Rosiglitazone |
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