| TRAIL联合吉西他滨对肺癌NCI-H460细胞增殖和凋亡的影响 |
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| 引用本文： | 孙非凡,孙铭娟,王梁华,焦炳华. TRAIL联合吉西他滨对肺癌NCI-H460细胞增殖和凋亡的影响[J]. 中国肿瘤生物治疗杂志, 2011, 18(3): 296-300. DOI: 10.3872/j.issn.1007-385X.2011.03.011 |
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| 作者姓名： | 孙非凡 孙铭娟 王梁华 焦炳华 |
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| 作者单位： | 1. 第二军医大学研究生管理大队,上海,200433
2. 第二军医大学基础部生物化学与分子生物学教研室,上海,200433 |
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| 基金项目： | 科技部“重大新药创制”科技重大专项（No. 2009ZX09103-689）；第二军医大学大学生创新能力培养研究课题资助项目（No. MS2009033） |
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| 摘 要： | 目的:观察吉西他滨(gemcitabine)联合TNF相关凋亡诱导配体(TNF-related apoptosis inducing ligand,TRAIL)对人肺癌细胞NCI-H460增殖和凋亡的影响。方法:MTT法检测NCI-H460细胞的增殖,流式细胞术检测NCI-H460细胞的凋亡。结果:吉西他滨和TRAIL单用或联用均可浓度(0.001~10μg/ml)和时间(24、48、72 h)依赖性抑制NCI-H460细胞的增殖,两药联用比单用时抑制率更高,3组药物作用72 h时对肿瘤的抑制率分别为14.9%~77.5%、23.4%~74.8%、22.3%~80.5%;作用72 h时两药单用和联用对细胞增殖抑制的IC50分别为0.085 3、0.098 2、0.043 5μg/ml。两药联用对NCI-H460细胞增殖的抑制效果还与用药顺序以及两药比例有关,吉西他滨作用24 h后再加TRAIL比TRAIL作用24 h后再加吉西他滨对NCI-H460的抑制效果更好,吉西他滨与TRAIL联用的比例为1∶0.3时对NCI-H460细胞增殖的抑制率最大。吉西他滨和TRAIL联用时NCI-H460细胞的凋亡率显著高于两药单用的凋亡率(49.04%vs29.33%、25.69%,P<0.01)。结论:吉西他滨与TRAIL联用可协同抑制肺癌细胞NCI-H460的增殖、促进细胞凋亡。
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| 关 键 词： | 吉西他滨 TNF相关凋亡诱导配体 人肺癌细胞 NCI-H460 增殖 凋亡 |
| 收稿时间： | 2011-04-10 |
| 修稿时间： | 2011-05-15 |
Effect of gemcitabine combined with TRAIL on proliferation and apoptosis of lung cancer NCI-H460 cells |
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| SUN Fei-fan,SUN Ming-juan,WANG Liang-hua and JIAO Bing-hua. Effect of gemcitabine combined with TRAIL on proliferation and apoptosis of lung cancer NCI-H460 cells[J]. Chinses Journal of Cancer Biotherapy, 2011, 18(3): 296-300. DOI: 10.3872/j.issn.1007-385X.2011.03.011 |
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| Authors: | SUN Fei-fan SUN Ming-juan WANG Liang-hua JIAO Bing-hua |
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| Affiliation: | Graduates Administration Brigade, Second Military Medical University, Shanghai 200433, China;Department of Biochemistry and Molecular Biology, Department of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China;Department of Biochemistry and Molecular Biology, Department of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China;Department of Biochemistry and Molecular Biology, Department of Basic Medical Sciences, Second Military Medical University, Shanghai 200433, China |
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| Abstract: | |
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| Keywords: | gemcitabine TNF-related apoptosis inducing ligand(TRAIL) human lung cancer cell NCI-H460 proliferastion apoptosis |
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