| 减毒水泡性口炎病毒诱导宫颈癌HeLa细胞的凋亡及其作用机制 |
| |
| 引用本文： | 连海,张静敏,夏志平,汤晶,张爽,于玲. 减毒水泡性口炎病毒诱导宫颈癌HeLa细胞的凋亡及其作用机制[J]. 中国肿瘤生物治疗杂志, 2011, 18(3): 264-269. DOI: 10.3872/j.issn.1007-385X.2011.03.05 |
| |
| 作者姓名： | 连海 张静敏 夏志平 汤晶 张爽 于玲 |
| |
| 作者单位： | 1. 军事医学科学院第十一研究所基础研究室,吉林长春,130062
2. 吉林大学药学院,吉林长春,130021 |
| |
| 基金项目： | 国家自然科学基金资助项目（No. 31001070）；吉林省科技发展计划项目（No. 20100146）；军事医学科学院第十一研究所创新启动基金资助项目(No.YCX0902) |
| |
| 摘 要： | 目的:研究减毒水泡性口炎病毒(vesicular stomatitis virus,VSV)对宫颈癌HeLa细胞的凋亡诱导作用,并探讨其可能的机制。方法:将减毒VSV以1.0 MOI的接种密度感染HeLa细胞,在6、12、18、24和30 h后收集细胞,MTT法检测HeLa细胞的增殖;AO/EB染色观察HeLa细胞凋亡形态学变化,Annexin V-FITC/PI双染法检测HeLa细胞早期凋亡率,流式细胞术分析HeLa细胞sub-G1凋亡峰,JC-1染色法测定细胞线粒体跨膜电位水平,caspase试剂盒检测HeLa细胞caspase-3、caspase-8及caspase-9的活性。结果:减毒VSV感染HeLa细胞12和24 h后,HeLa细胞增殖活力分别为(78.4±1.9)%和(63.1±5.6)%(P<0.01);早期凋亡细胞率分别为(16.88±2.48)%和(31.9±4.24)%(P<0.01),sub-G1凋亡峰分别为(14.85±1.48)%和(21.05±2.28)%(P<0.01)。随着减毒VSV感染时间的增加,HeLa细胞线粒体跨膜电位逐渐降低(P<0.05),caspase-9和caspase-3的活性显著升高(均P<0.05)。结论:减毒VSV能够抑制HeLa细胞的增殖,并通过caspase-9和caspase-3依赖的途径诱导HeLa细胞凋亡。
|
| 关 键 词： | 水泡性口炎病毒 宫颈肿瘤 HeLa细胞 凋亡 |
| 收稿时间： | 2011-02-20 |
| 修稿时间： | 2011-04-15 |
Attenuated vesicular stomatitis virus induces apoptosis in cervical cancer HeLa cells and its mechanism |
| |
| LIAN Hai,ZHANG Jing-min,XIA Zhi-ping,TANG Jing,ZHANG Shuang and YU Ling. Attenuated vesicular stomatitis virus induces apoptosis in cervical cancer HeLa cells and its mechanism[J]. Chinses Journal of Cancer Biotherapy, 2011, 18(3): 264-269. DOI: 10.3872/j.issn.1007-385X.2011.03.05 |
| |
| Authors: | LIAN Hai ZHANG Jing-min XIA Zhi-ping TANG Jing ZHANG Shuang YU Ling |
| |
| Affiliation: | Department of Basic Sciences, Eleventh Institute of Academy of Military Medical Sciences, Changchun 130062, Jilin, China;School of Pharmacy, Jilin University, Changchun 130021, Jilin, China;Department of Basic Sciences, Eleventh Institute of Academy of Military Medical Sciences, Changchun 130062, Jilin, China;School of Pharmacy, Jilin University, Changchun 130021, Jilin, China;Department of Basic Sciences, Eleventh Institute of Academy of Military Medical Sciences, Changchun 130062, Jilin, China;Department of Basic Sciences, Eleventh Institute of Academy of Military Medical Sciences, Changchun 130062, Jilin, China |
| |
| Abstract: | Objective: To investigate the apoptosis-inducing effect of an attenuated vesicular stomatitis virus in cervical cancer HeLa cells,and to explore the possible mechanism.Methods: HeLa cells were infected with VSV(MOI=1.0) and the cell proliferation was determined by MTT assay at 6,12,18,24,and 30 h after infection.Morphological changes of apoptosis were observed by acridine orange(AO)/ethidium bromide(EB) staining.Annexin V/PI double-staining was performed to detect early apoptosis rate of HeLa cells and the ... |
| |
| Keywords: | vesicular stomatitis virus (VSV) cervical cancer HeLa cell apoptosis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国肿瘤生物治疗杂志》浏览原始摘要信息 |
|
点击此处可从《中国肿瘤生物治疗杂志》下载免费的PDF全文 |
|
