| 慢性乙肝患者树突状细胞抗原递呈功能状态的初步研究 |
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| 引用本文： | 李声方,赵登蕴,刘贵章,覃亚勤,左海军,欣文.慢性乙肝患者树突状细胞抗原递呈功能状态的初步研究[J].医学信息,2011(2). |
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| 作者姓名： | 李声方 赵登蕴 刘贵章 覃亚勤 左海军 欣文 |
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| 作者单位： | 中山大学附属东莞东华医院感染科; |
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| 基金项目： | 广东省卫生厅科研基金项目(No:WSTJJ20061225420300197302210916) |
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| 摘 要： | 目的:研究慢性乙肝患者树突状细胞(DC)抗原递呈功能的改变。方法:从慢乙肝患者和健康人外周血分离单个核细胞,诱导培养出DC,显微镜下观察DC形态并计数,流式细胞仪检测DC表面协同刺激分子(CD80,CD86),MTT法检测DC刺激同种异体淋巴细胞增殖的能力,ELISA法测定培养上清液中IL-12、r-IFN水平。结果:慢乙肝患者外周血单个核细胞诱导培养所获得的DC数量及表面协同刺激分子(CD80,CD86)表达水平、刺激同种异体淋巴细胞增殖能力和细胞因子产生水平均明显低于健康者(P<0.05)。结论:慢乙肝患者DC的数量及抗原递呈、免疫刺激功能状态均处于低下水平。
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| 关 键 词： | 慢性乙型肝炎 树突状细胞 |
Study on the functional status about antigen presenting of Dendritic Cells in patients with chronic hepatitis B |
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| LI Sheng-fang,ZHAO Deng-yun,LIU Gui-zhang,et al DongHua Hospital of Zhong Shan University,DongGuan ,China.Study on the functional status about antigen presenting of Dendritic Cells in patients with chronic hepatitis B[J].Medical Information,2011(2). |
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| Authors: | LI Sheng-fang ZHAO Deng-yun LIU Gui-zhang DongHua Hospital of Zhong Shan University DongGuan China |
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| Institution: | LI Sheng-fang,ZHAO Deng-yun,LIU Gui-zhang,et al DongHua Hospital of Zhong Shan University,DongGuan 523110,China |
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| Abstract: | Objective:To study the capacity about antigen presenting of Dendritic Cells(DC) in patients with chronic hepatitis B.Methods:Monocytes were isolated from peripheral blood of CHB and healthy individuals and incubated to induce DC generation.The morphotype of DC was identified by microscope.The phenotype of DC(CD80,CD86) was characterized by flow cytometry.The capacity of stimulating reaction of allogenic T lymphocytes was detected by MTT assay.The concentration of cytokines (IL-12,r-IFN) in supernate was tes... |
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| Keywords: | Chronic Hepatitis B(CHB) Dendritic cells(DC) |
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