| 树脂吸附法纯化橙皮中橙皮苷的工艺研究 |
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| 引用本文： | 陈竹,张保顺,叶小利,孙胜亮,李学刚.树脂吸附法纯化橙皮中橙皮苷的工艺研究[J].重庆医学,2011,40(15):1502-1504. |
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| 作者姓名： | 陈竹 张保顺 叶小利 孙胜亮 李学刚 |
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| 作者单位： | 陈竹,Chen Zhu(重庆医药高等专科学校药学系,400030);张保顺,李学刚,Zhang Baoshun,Li Xuegang(西南大学药学院,重庆,400716);叶小利,孙胜亮,Ye Xiaoli,Sun Shengliang(西南大学生命科学学院,重庆,400716) |
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| 基金项目： | 重庆市医学科研计划项目，中央高校基本科研业务费专项资金资助项目 |
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| 摘 要： | 目的研究大孔树脂吸附法分离纯化橙皮中橙皮苷的最佳工艺条件,通过熔点、橙皮苷的薄层(TLC)、UV及IR图谱进行结构表征。方法以橙皮苷的纯度为指标,比较HPD826、HPD417、AB-8、HPD722及HPD600大孔树脂对橙皮苷的吸附和解吸附性能。结果 AB-8大孔树脂对橙皮苷的吸附和解吸附性能较好,吸附量为0.37 mg/g,吸附率为86.2%;10%的乙醇碱溶液(V/V)洗脱,解吸率为72.7%。结论橙皮苷的最佳纯化工艺是选用AB-8大孔树脂吸附,pH12的10%乙醇碱溶液(V/V)洗脱,所得产物纯度达91.5%,同时图谱确证了橙皮苷的结构。
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| 关 键 词： | 橙皮苷 大孔树脂 橙皮 纯化 |
Purification of hesperidin from Pericarpium citri reticulatae with macroporous resin |
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| Chen Zhu,Zhang Baoshun,Ye Xiaoli,Sun Shengliang,Li Xuegang.Purification of hesperidin from Pericarpium citri reticulatae with macroporous resin[J].Chongqing Medical Journal,2011,40(15):1502-1504. |
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| Authors: | Chen Zhu Zhang Baoshun Ye Xiaoli Sun Shengliang Li Xuegang |
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| Institution: | Chen Zhu1,Zhang Baoshun2,Ye Xiaoli3,Sun Shengliang3,Li Xuegang2(1.Department of Pharmacy,Chongqing Medical and Pharmaceutical College,Chongqing 400030,China,2.College of Pharmaceutical Science,Southwest University,Chongqing 400716,3.College of Life Science,China) |
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| Abstract: | Objective To study the optimally purified process of hesperidin extracted from Pericarpium citri reticulatae with macroporous resin and to confirm the structure of hesperidin by melting point(M.P.),TLC,UV and IR spectra data.Methods For the purity of hesperidin as a main objective,the adsorption and desorption characters of HPD826,HPD417,AB-8,HPD722 and HPD600 macroporous resins were compared.Results AB-8 had the best adsorptive property and desorption,the adsorptive content of AB-8 was 0.37 mg/g.The adsorp... |
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| Keywords: | hesperidin microporous resin Pericarpium citri reticulatae purification |
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