| 重庆地区汉族CD14启动子区多态性分析 |
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| 引用本文： | 冯凯,刘兴,蒋建新.重庆地区汉族CD14启动子区多态性分析[J].第三军医大学学报,2004,26(18):1667-1669. |
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| 作者姓名： | 冯凯 刘兴 蒋建新 |
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| 作者单位： | 第三军医大学大坪医院野战外科研究所第四研究室,创伤、烧伤与复合伤国家重点实验室,重庆,400042;第三军医大学大坪医院野战外科研究所第四研究室,创伤、烧伤与复合伤国家重点实验室,重庆,400042;第三军医大学大坪医院野战外科研究所第四研究室,创伤、烧伤与复合伤国家重点实验室,重庆,400042 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 目的调查CD14(-260)多态性在重庆地区汉族中的分布特征,及检验该随机样本遗传学研究的可靠性.方法对110名重庆汉族个体,采用降落式PCR和限制性片段长度多态性法(RFLP)进行基因型分析.结果重庆地区汉族CD14(-260)多态性TT、TC、CC 3种基因型频率分别为43.6%、39.1%和17.3%; 样本分布符合Hardy-Weinberg平衡.结论重庆汉族CD14(-260)多态性基因型分布特征与白种人存在明显差异.样本为H-W平衡群体,可进一步用于重庆汉族全身性炎症反应的关联分析.
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| 关 键 词： | 降落式PCR(TD-PCR) 限制性片段长度多态性(RFLP) CD14 |
| 文章编号： | 1000-5404(2004)18-1667-03 |
| 修稿时间： | 2003年6月23日 |
Polymorphism in CD14 promoter region in Han population in Chongqing |
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| FENG Kai,LIU Xing,JIANG Jian xin.Polymorphism in CD14 promoter region in Han population in Chongqing[J].Acta Academiae Medicinae Militaris Tertiae,2004,26(18):1667-1669. |
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| Authors: | FENG Kai LIU Xing JIANG Jian xin |
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| Abstract: | Objective To investigate the distribution of CD14C ( 260)T single nucleotide polymorphism (SNP) in the Han population in Chongqing and to test the reliability with this random sample in genetic research. Methods Single round Touchdown PCR (TD PCR) and restriction fragment length polymorphism (RFLP) method were used for genotyping in 110 people from the Han population in Chongqing. Results Frequencies of TT, CT, and CC genotypes were 43 6%, 39 1%, and 17 3%, respectively. The sample distribution was accorded with Hardy Weinberg principle. Conclusion CD14C( 260)T SNP was found in the Han population in Chongqing, and the distribution of alleles was significantly different from that in white population. The sample from an H W equilibrium population can be used in the association study of the systematic inflammatory reaction in the Han population in Chongqing. |
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| Keywords: | CD14 |
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