羧甲基壳聚糖对雌二醇凝胶透皮吸收促进作用的研究

目的：研究羧甲基壳聚糖（CMC）对雌二醇凝胶体外透皮吸收的影响。方法：合成CMC，用电位滴定法确定其取代度。以含2％CMC的雌二醇凝胶为试验组，含2％氮酮的雌二醇凝胶为阳性对照组，无任何促透剂的雌二醇凝胶为阴性对照组，通过体外透皮扩散实验得到各组累积透过量，并求出稳态透皮速率。结果：合成得到的CMC取代度为0．52。试验组的累积透过量和稳态透皮速率显著大于阴性对照组（P〈0．05），但与阳性对照组相比，差异无统计学意义（P〉0．05）。结论：2％CMC与2％氮酮的促透效果相似，值得进一步研究。

Study on the Transdermal Permeation Enhancing Effect of Carboxymethyl Chitosan on Estradiol Gels in vitro

Objective ： To study the enhancing effect of carboxymethyl chitosan （ CMC ） on the estradiol gel in vitro. Method： CMC was synthesized and the substitution degree was determined by potentiometric titration. Three different kinds of estradiol gels, which was with 2% CMC （testing group） , 2% azone （ positive control group） and without any enhancer （ negative control group） , respectively, were studied. The cumulative penetration （ Q ） and steady penetration rate（ J ） in vitro were used as the indices. The cumulative penetration was calculated. Result ： The substitution degree of CMC was 0.52. Significant difference was found on the cumulative penetration and the steady penetration rate of testing group when compared with negative control group （ P 〈 0.05 ） , however no significant difference was found when testing group was compared with positive control group （ P 〉 0.05）. Conclusion： 2% CMC was an efficient penetration enhancer with similar effect with 2% azone and valuable to be studied further.