| 富血小板血浆PRP的体外骨诱导作用研究 |
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| 引用本文： | 张秀白,刘宏伟,刘兴文,李毅.富血小板血浆PRP的体外骨诱导作用研究[J].临床口腔医学杂志,2004,20(5):291-293. |
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| 作者姓名： | 张秀白 刘宏伟 刘兴文 李毅 |
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| 作者单位： | 第一军医大学附属南方医院口腔科,广东,广州,510515 |
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| 基金项目： | 全军十五医药卫生科研基金资助 (0 1L0 75)，广东省科技计划项目资助 (C30 70 4 ) |
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| 摘 要： | 目的 :探讨在体外培养中富血小板血浆 (Platelet -RichPlasma ,PRP)对骨髓基质细胞诱导成骨的影响。方法 :从自体静脉血中提取PRP ,配制成条件培养液 ,并作用于培养状态的骨髓基质细胞 ,染色检测细胞碱性磷酸酶表达情况 ,钙化结节形成率 ,放免法测定培养液中骨钙素的含量。结果 :骨髓基质细胞经诱导培养后 ,碱性磷酸酶、骨钙素表达明显增加。结论 :体外培养时 ,PRP可促进骨髓基质细胞的成骨分化。
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| 关 键 词： | 富血小板血浆 骨髓基质细胞 骨钙素 |
| 文章编号： | 1003-1634(2004)05-0291-03 |
The study of Platelet-rich plasma on differentiation of bone marrow stromal cells into osteoblasts in vitro |
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| ZHANG Xiu-bai,LIU Hong-wei,LIU Xing-wen,LI Yi.The study of Platelet-rich plasma on differentiation of bone marrow stromal cells into osteoblasts in vitro[J].Journal of Clinical Stomatology,2004,20(5):291-293. |
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| Authors: | ZHANG Xiu-bai LIU Hong-wei LIU Xing-wen LI Yi |
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| Institution: | ZHANG Xiu-bai,LIU Hong-wei,LIU Xing-wen,LI Yi.Department of Stomatology,the First Military Medical University,Affliated Nanfang Hospital.Guangzhou 510515,China |
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| Abstract: | Objective:To explore the effects of Platelet-rich plasma(PRP) on the induced osteoblast of bone marrow stromal cells(BMSCs).Method: PRP were extracted from autologous venous blood and then dissolved into DMEM medium. BMSCs were cultured with the prepared special medium, and then stained for alkaline phosphatase (ALP) , measured for the amount of osteocalcin in the culture medium. Result:After BMSCs were cultured with the prepared medium,the expressions of ALP and the amount of osteocalcin were increased greatly.Conclusion:The results show PRP can promote BMSCs differentiated into osteoblasts in vitro. |
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| Keywords: | platelet-rich plasma(PRP) bone marrow stromal cells osteocalcin |
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