鸡血藤醇提物对血虚模型小鼠的补血作用

目的:研究鸡血藤醇提物对血虚模型小鼠的补血作用。方法:通过放血以复制小鼠失血性血虚模型;通过腹腔注射环磷酰胺(25 mg/kg)以复制小鼠化学性血虚模型;通过皮下注射乙酰苯肼(20 mg/kg)同时控制饮食以复制小鼠综合血虚模型。60只KM种小鼠随机分为正常对照(等体积生理盐水)组、模型(等体积生理盐水)组、乌鸡白凤丸(4 g/kg)组与鸡血藤醇提物高、中、低剂量(30、15、7.5 g/kg)组。制备模型的同时灌胃给药,每天1次,连续10 d。测定小鼠红细胞计数(RBC)、血红蛋白(HGB)、红细胞压积(HCT)、血小板计数(PLT)。结果:与正常对照组比较,模型组小鼠RBC、HGB、HCT、PLT降低,差异具有统计学意义(P<0.01)。与模型组比较,鸡血藤醇提物高、中、低剂量组小鼠RBC、HGB升高,差异具有统计学意义(P<0.01或P<0.05);鸡血藤醇提物高、中剂量组小鼠HCT、PLT升高,差异具有统计学意义(P<0.01或P<0.05)。结论:鸡血藤醇提物对血虚模型小鼠有良好的补血作用,其机制可能与调节血液系统有关。

Effects of Ethanol Extract ofSpatholobus suberectus on Blood Deficiency Model Mice

OBJECTIVE： To study the effects of ethanol extract of Spatholobus suberectus on blood deficiency model mice. METHODS： Hemorrhagic blood deficiency model was induced by bloodletting; the chemical deficiency model was induced by intraperitoneal injection of cyclophosphamide （25 mg/kg） ; comprehensive blood deficiency model was induced by subcutaneous injection of acetylphenylhydrazine （20 mg/kg） and controlling diet. 60 KM mice were randomized into normal control group （constant volume of normal saline）, model group （constant volume of normal saline）, Wuji baifeng pills group （4 g/kg） and ethanol extract of S. suberectus high-dose, medium-dose and low-dose groups （30, 15, 7.5 g/kg）. Blood deficiency model was induced and given relevant medicines intragastrically once a day for consecutive 10 days. RBC, HGB, HCT and PLT were determined. RESULTS： Compared with normal control group, the levels of RBC, HGB, HCT and PLT were decreased significantly in model group（P〈 0.01） ; compared with model group, RBC and HGB in ethanol extract of S. suberectus high-dose, medium-dose and low-dose groups were increased significantly（P〈0.01 or P〈0.05）. HCT and PLT in ethanol extract of S. suberectus high-dose and medium-dose groups were increased significantly（P〈0.01 or P〈0.05）. CONCLUSIONS： The ethanol extract of S. suberectus has good effects on blood deficiency model mice, and the mechanism may be related to the regulation of blood system.