肠血管活性多肽对多器官功能障碍综合征大鼠肠淋巴细胞归巢的影响

为观察肠血管活性多肽 (VIP )对大鼠肠缺血再灌注致多器官功能障碍综合征 (MODS )时 ,肠淋巴细胞归巢至肠相关淋巴细胞 (GALT )的调节以及对MODS转归的影响。本研究用微型无创动脉夹夹住大鼠肠系膜动脉根部 4 5min ,松夹后再灌注6h ,制备MODS模型。将VIP分别从股静脉输入和从腹腔注入大鼠体内。收集肠淋巴液 ,计数淋巴细胞总数及T、B淋巴细胞比例 ,了解淋巴细胞进入血循环状况。体外用51Cr标记肠淋巴细胞 ,回输入大鼠体内 ,用γ计数器检测各器官组织内的51Cr 肠淋巴细胞 ,了解肠淋巴细胞归巢。检测血及肠淋巴TNF α、内毒素 ;测定血D 乳酸、谷丙转氨酶 (ALT )、肌肝 (Cr)、血氧分压 ;观察重要器官组织学改变 ,评价VIP对MODS时各脏器形态及功能改变。结果显示 ,VIP使MODS大鼠肠粘膜迁移至血循环肠淋巴细胞总数 [(0 4 2± 0 18)× 10 7/h]较未予处理的MODS组 [(0 2 8± 0 15 )× 10 7/h]显著增加 (P <0 0 5 ) ,以T细胞数上升明显。VIP减少了MODS大鼠归巢至肠粘膜的肠淋巴细胞 ,Peyer淋巴结及小肠分布的51Cr 细胞量分别占总51Cr量的 2 14 %± 1 4 9%、 1 5 8%± 0 4 2 % ,显著低于未予处理的MODS组 (5 0 4 %± 1 2 3%和 3 2 3%± 1 6 9% ,P <0 0 1及P <0 0 5 )。外源性给予MODS大鼠VIP后 ,肠淋巴

The Effect of Vasoactive Intestinal Peptide on Homing of Intestinal Lymphocytes in Rats with Multiple Organ Dysfunction Syndrome

To observe the effect of vasoactive intestinal peptide (VIP) on intestinal lymphocytes homing at gut-associated lymphoid tissues (GALT) in rats with multiple organs dysfunction syndrome (MODS),the superior mesenteric artery was occluded for 45 minutes and reperfused for 6 hours to make the model of MODS. VIP was infused into rats from femoral vein and from abdomen. Intestinal lymph was collected to measure the population of lymphocytes per hour and the percentage of T and B cells. In addition,the intestinal lymphocytes from intestinal lymph were labeled with 51 Cr and then infused into femoral vein of rats with MODS. 51 Cr-intestinal lymphocytes in intestinal mucosa and Peyer's patches were counted with γ counter. The level of endotoxin and TNF-α in intestinal lymph and D-(-)-lactate acid,ALT,cretinine,PaO 2 in plasma was determined,and the histology of essential organs was compared between MODS and MODS treated with VIP. The results showed that VIP increased the population of intestinal lymphocytes [(0.42±0.180×10 7/h vs (0.28±0.15)×10 7/h, P <0.05] trafficked from intestinal mucosa into blood circulation in rats with MODS. The percentage of 51 Cr-intestine lymphocytes in Peyer's patches (2.14%±1.49%) and intestine mucosa (1.58%±0.42%) of rats with MODS treated with VIP was remarkably decreased in compared with those of rat with MODS (5.04%±1.23%, P <0.01,3.23%±1.69%, P <0.05). The endotoxin or TNF-α level of intestine lymph and D-(-)-lactate acid,ALT and cretinine in plasma of rats with MODS was greatly decreased after in fusion of VIP PaO2 was increased to 4 9%,and the severe inflammation in the essential organs was relieved It condudes that the decrement of endotoxin or TNF αand improvement of the function of each organ might be related with decline of intestinal lymphocytes homing to GALT caused by VIP that relieved the severe inflammation in intestinal mucosa