BMP-2基因转染兔骨骼肌干细胞修复骨缺损实验研究

目的 探讨AdrhBMP-2修饰的兔骨骼肌干细胞(RRSMSCs)为种子细胞、脱钙骨基质(DBM)为支架材料构建组织工程化人工骨修复兔桡骨大段骨缺损的可行性。方法 建立兔桡骨干骨缺损(20mm)模型，50只新西兰兔分为5组：AdrhBMP-2转染RSMSCs／DBM组(A组)、AdGFP转染RSMSCs／DBM组(B组)、未转染RSMSCs／DBM组(C组)、单纯DBM组(D组)和对照组(E组)。术后4周和6周分别行骨密度、X线、生物力学及组织学检查。结果 术后4周，A组骨修复达到X线愈合标准，但髓腔不通；术后6周，各组的愈合率分别为A组100％、B组50%、C组33％，D组和E组为0。结论 AdrhBMP-2修饰的RSMSCs为种子细胞、DBM为支架材料构建的基因强化组织工程骨能修复兔桡骨长20mm的大段骨缺损，为临床上大范围骨缺损的修复提供了一种更为有效的方法和材料。

Study of repair of bone defect using rabbit skeletal muscle stem cells transduced by AdrhBMP-2 gene

ObjectiveTo observe the effect of rabbit skeletal muscle stem cells (RSMSCs) modified by adenovirus mediated bone morphogenetic protein-2 (BMP-2) gene ex vivo in combination with demineralized bone matrix (DBM) on repair of longer bone defect in rabbit.MethodsModel of radial bone defects (20 mm) of rabbits was established. 50 rabbits were divided into 5 groups, group A (AdrhBMP-2 trusduced RSMSCs/DBM group), group B (adGFP trusduced RSMSCs/DBM group), group C (not trusduced RSMSCs/DBM group), group D (DBM group), and group E (untreated group). Roentgenographic, histologic, biomechanical, bone density of all animals were examined at the end of 4th and 6th week after surgery.ResultsAt 4th week, radial bone defects healed in group A. The healing rates from group A to group E were 100%, 50%, 33%, 0%, and 0% respectively at 6th week.ConclusionRSMSCs modified by AdrhBMP-2 ex vivo in combination with DBM can repair radial longer segemental defect in rabbit. It's possible to be used in the clinical treatment of longer segemental bone defect.